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331569
Sigma-AldrichEthidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
More>>Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Less<<
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Description
Overview
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Catalogue Number
331569
Brand Family
Calbiochem®
Application Data
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
References
References
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Physicochemical Information
Cell permeable
N
Safety Information
R Phrase
R: 36
Irritating to eyes.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Global Trade Item Number
Bestellnummer
GTIN
331569-1EA
04055977195750
Documentation
Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem SDB
Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem Analysenzertifikate
Titel
Chargennummer
331569
Literatur
Übersicht
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Datenblatt
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-December-2010 RFH
Application Data
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
Description
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Form
Gray-brown suspension
Recommended reaction conditions
Ethidium bromide (EtBr) is the fluorescence dye most frequently used to stain nucleic acids in agarose and polyacrylamide gels. It intercalates primarily with double-stranded DNA to form an orange-red fluorescent complex under the influence of UV light. This interaction with nucleic acids makes ethidium bromide potentially mutagenic and is classified as toxic.
Capacity
The column capacity is at least 300 mg EtBr, corresponding to 30 L gel staining solution. The binding capacity of the cartridge is tested with a 10 mg/L EtBr solution of 1X TAE buffer; >99% of the dye was adsorbed up to the 300 mg limit as measured by fluorescence spectrometry.
Flow Rate
The flow rate of the adsorber column depends on the volume and height of the reservoir used for the staining solution and is a minimum of 200 ml/hr.
Connecting the Adsorber Column
The cartridge, as supplied, is sealed at both ends with small plastic caps (see figure 1). These caps should be removed prior to use and the cartridge connected to a reservoir containing the ethidium bromide solution to be adsorbed. A glass or plastic reservoir fitted with a stopcock or tubing clamp should be used to that the column can be switched on and off as required. To avoid any blocking of the frits with gel particles in the staining solutions, it is advisable to put some glass wool, filter paper, etc. in the connecting tube. If the flow rate of the adsorber column is distinctly lower than 1-2 drops per second, residual air bubbles must be removed by repeatedly short-squeezing the tubing above the cartridge. To guarantee a good flow rate throughout the lifespan of the cartridge, the formation of air bubbles in the system should be avoided, as these tend to hinder flow. The residual capacity of the column is easy to assess visually as the ethidium bromide moves through the column in the form of an increasingly dark red to black band. As long as this band has not reached the bottom of the column, the concentration of dye is still below the specified detection limit.
Figure 1: Ethidium Bromide Adsorber
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
Disposal
Once the capacity has been exhausted, the adsorber column should be disposed of according to local regulations.
CAS number
69011-20-7
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Toxicity
Irritant
References
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
