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324682 Elastase, Porcine Pancreas, High Purity, Crystallized

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324682
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Preis & Verfügbarkeit

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324682-1000U
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      Kst.-Ampulle 1000 u
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      324682-250U
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      Eingeschränkte Verfügbarkeit
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      Lieferbar 
      Produkt wurde eingestellt
      Begrenzter Lagerbestand
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          Contact Customer Service

          Kst.-Ampulle 250 u
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          Die Mindestmenge muss ein Vielfaches sein von
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          Description
          OverviewNative elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
          Catalogue Number324682
          Brand Family Calbiochem®
          References
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Product Information
          CAS number39445-21-1
          Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-<i>p</i>NA (<a href = "/Products/ProductDisplay.asp→catNO=324699">Cat. No. 324699</a>) per min at 25°C, pH 8.0.
          EC number3.4.21.36
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          Quality LevelMQ100
          Applications
          Biological Information
          Specific Activity≥25 units/mg protein
          Physicochemical Information
          ContaminantsChymotrypsin: ≤0.2%
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSTS5052000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Bestellnummer GTIN
          324682-1000U 04055977196832
          324682-250U 07790788054380

          Documentation

          Elastase, Porcine Pancreas, High Purity, Crystallized SDB

          Titel

          Sicherheitsdatenblatt (SDB) 

          Elastase, Porcine Pancreas, High Purity, Crystallized Analysenzertifikate

          TitelChargennummer
          324682

          Literatur

          Übersicht
          Largman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.
          Datenblatt

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-July-2010 JSW
          DescriptionNative elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
          FormLyophilized
          FormulationLyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
          CAS number39445-21-1
          RTECSTS5052000
          EC number3.4.21.36
          ContaminantsChymotrypsin: ≤0.2%
          Specific activity≥25 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0.
          SolubilitydH₂O (10 mg/ml)
          Storage Protect from moisture
          -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
          Toxicity Standard Handling
          Merck USA index14, 3535
          ReferencesLargman, C. et al. 1976. Biochemestry 15, 2491.
          Mandl, I. 1962. Methods in Enzymol. 5, 665.

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          Kategorien

          Life Science Research > Proteins and Enzymes > Other Enzymes