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ESG2207
Sigma-AldrichESGRO® Recombinant Rat LIF Protein
ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml
More>>ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml Less<<
ESGRO® Recombinant Rat LIF Protein: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE.
Description
Catalogue Number
ESG2207
Brand Family
Chemicon®
Trade Name
Chemicon
Description
ESGRO® Recombinant Rat LIF Protein
Overview
Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.
Alternate Names
Rat LIF
References
Product Information
Format
Purified
HS Code
3002 15 90
Presentation
Purified, sterile liquid. Rat ESGRO® is supplied in liquid form as 107 Units ESGRO® in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.
Rat ESGRO® is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.
ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml
Key Applications
Stem Cell Culture
Application Notes
Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et al. 1998).
Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et al. 1998).
At this recommended concentration 106 units of ESGRO® is sufficient for 1L of tissue culture media.
Visit www.esgro-lif.com for additional information
Biological Information
Concentration
10 million units/1 mL
Purity
The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE.
Source
rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Specific Activity
Rat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells. A standard of 50 Units is defined as the concentration of Rat ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies. 10<sup>6</sup> units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. <br /> Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity > 10<sup>8</sup> units/mg. <br /><br /> Note: LIF should not be allowed to come in contact with ruminant or swine.
Entrez Gene Summary
Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22008 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Media Form
Liquid
Stem Cell Type
Mouse Embryonic Stem Cells
Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Rat ESGRO® is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells.
For long term storage it is recommended that Rat ESGRO® concentrate be stored at -20°C.
It is recommended that prior to use, Rat ESGRO® should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended.
Background: Recent progress in rat pluripotent stem cell technology has been remarkable. Particularly salient is the demonstration that embryonic stem cells (ESCs) in the rat (rESCs) can contribute to germline transmission, permitting generation of gene-modified rats as is now done using mouse ESCs (mESCs) or mouse induced pluripotent stem cells (iPSCs; miPSCs). However, determinations of whether rat iPSCs (riPSCs) can contribute to germ cells are not published. Here we report the germline competency of riPSCs.
Establishment of rat embryonic stem cell lines that can participate in germline chimerae at high efficiency. Hirabayashi M. et al. Mol. Reprod. Dev.
77(2)
94
2009
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat embryonic stem (rES) cell is an important resource for the study of disease models. Attempts to derive ES cells from various mammals, including the rat, have not succeeded. Here we have established two independent rES cells from Wister rat blastocysts that have undifferentiated characters such as Nanog and Oct3/4 genes expression and they have stage-specific embryonic antigen (SSEA) -1, -3, -4, and TRA-1-81 expression. The cells were successfully cultured in an undifferentiated state and can be possible over 18 passages with maintaining more than 40% of normal karyotype. Their pluripotent potential was confirmed by the differentiation into derivatives of the endoderm, mesoderm, and ectoderm. Most importantly, the rES cells are capable of producing chimera rats. Therefore, we established pluripotent rES cell lines that are widely used to produce genetically modified experimental rats for study of human diseases.
