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HTS017M ChemiSCREEN™ C5aR Membrane Preparation, recombinant

Human C5aR GPCR membrane preparation for Radioligand binding Assays.

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Host Cell: Chem-1 Uni Prot Number: P21730 Entrez Gene Number: NM_001736.3
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HTS017M
200 assays  
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      Übersicht

      Replacement Information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1C5aR
      Description
      Catalogue NumberHTS017M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ C5aR Membrane Preparation, recombinant
      OverviewFull-length human C5aR cDNA
      Background InformationC5a is a proinflammatory peptide generated through activation of the complement system, and is more potent than the other anaphylatoxins, C4a and C3a, in activating peripheral blood leukocytes (Gerard & Gerard, 1994). It can actively participate in the regulation of local cytokine network by stimulating production of proinflammatory cytokines (Buchner et al., 1995; Hsu, et al., 1999). The proinflammatory effects of C5a are mediated through binding to a specific 7-TM chemoattractant receptor, C5aR (CD88). Binding of C5a to phagocyte C5aR induces chemotaxis, production of superoxide anions, and release of degradative enzymes. Pharmacologic or genetic disruption of C5a/C5aR interaction reduces sepsis (Riedemann et al., 2003), immune complex-induced lung disease (Shushakova et al., 2002), and Arthrogen-induced arthritis (Grant et al., 2002) in experimental models. Chemicon's cloned human C5a receptor membrane preparations are ideal tools for screening for antagonists of C5a/C5aR interactions. The C5aR Membrane Preps bind specifically to 125I-labeled ligand C5a, with Kd of 0.59 nM. With 5 μg membrane per well and 0.1 nM 125I-labeled C5a, more than 15-fold signal:background was obtained.
      References
      Product Information
      FormatMembranes
      PresentationMembranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 1000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of I-125 labeled C5a (0.1 nM input concentration).
      Quality LevelMQ100
      Applications
      ApplicationHuman C5aR GPCR membrane preparation for Radioligand binding Assays.
      Key Applications
      • Radioligand binding assay
      Application NotesRadioligand binding assay (See images & graphs link)
      Biological Information
      Concentration0.5 mg/mL
      Entrez Gene Number
      Gene Symbol
      • C5AR1
      • CD88
      • C5AR
      • C5A
      • C5R1
      • C5aR
      • C5a-R
      GPCR ClassA
      Host CellsChem-1
      Protein TargetC5aR
      Target Sub-FamilyAnaphylotoxin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P21730 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
      SIZE: 350 amino acids; 39321 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
      SIMILARITY: SwissProt: P21730 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSPECIFICATIONS: Bmax for [125I]C5a binding: 9.7 pmol/mg protein
      Kd for [125I] C5a binding: ~ 0.59 nM
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
      Packaging Information
      Material Size200 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      HTS017M 04053252328473

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      Kategorien

      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
      Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations