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SCC165 BLaER1 Human B-cell Precursor Leukemia Cell Line

BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.

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Key Applications: Cell Culture
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SCC165
≥1X10⁶ cells/vial   
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      Übersicht

      Replacement Information

      Key Spec Table

      Key Applications
      CULT
      Description
      Catalogue NumberSCC165
      DescriptionBLaER1 Human B-cell Precursor Leukemia Cell Line
      Alternate Names
      • B cell Leukemia C/EBPalphaER clone 1
      Background InformationInduction of differentiation is a therapeutic approach for cancer. Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective (1). Ectopic expression of the transcription factor C/EBPα causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype.

      The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPα fused with the estrogen receptor hormone binding domain (ER) and GFP (2,3). A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages (2). Cells retained their phenotype even when C/EBPα was inactivated, a characteristic of genuine cell reprogramming. C/EBPα induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.

      Source:
      BLaER1 cells are a single subclone isolated from C/EBPαER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 (4).

      Biosafety Level 2:
      BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.

      References:
      1. Waxman, S. (2000). Leukemia 14(3):491-496.
      2. Rapino F et al. (2013). Cell Reports 3(4): 1153-1163.
      3. Rapino F et al. (2017). Cell Reports 19(6): 1281.
      4. Jack I et al. (1986). Cancer Genet. Cytogenet. 19(3-4):261-269.
      References
      Product Information
      Applications
      ApplicationBLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      • Cells tested positive for squirrel monkey retrovirus (SMRV).
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCC165 04054839573750

      Documentation

      BLaER1 Human B-cell Precursor Leukemia Cell Line SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Literatur

      ÜbersichtPub Med ID
      CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response
      Grégoire Stik, <br />Enrique Vidal, <br />Mercedes Barrero, <br />Sergi Cuartero, <br />Maria Vila-Casadesús, <br />Julen Mendieta-Esteban, <br />Tian V. Tian, <br />Jinmi Choi, <br />Clara Berenguer, <br />Amaya Abad, <br />Beatrice Borsari, <br />François le Dily, <br />Patrick Cramer, <br />Marc A. Marti-Renom, <br />Ralph Stadhouders & <br />Thomas Graf
      Nature Genetics  2  655–661  2020

      		32514124 32514124

      Datenblatt

      Titel
      BLaER1 Human B-cell Precursor Leukemia Cell Line