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14-911 Aurora C Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end. For use in Kinase Assays.

Aurora C Protein, active, 10 µg: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Key Applications: Kinase Assay UniProt Number: Q9UQB9
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14-911
10 µg  Also available in 250µg size as catalog # 14-911M - Call for pricing and availability.
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      Übersicht

      Replacement Information

      Key Spec Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      KANP_001015878 Human Q9UQB9
      Description
      Catalogue Number14-911
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAurora C Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end.
      Combined purity 94% by SDS-PAGE and Coomassie blue staining. Aurora C MW = 36kDa, INCENP MW = 38kDa.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceCo-expressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in
      organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
      Gene Symbol
      • AIK32 3
      • ARK-3
      • STK13
      • ARK3
      • AurC
      Purification MethodNi2+/NTA agarose
      UniProt Number
      UniProt SummaryFunction: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
      Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
      Cofactor: Magnesium.
      Enzyme regulation: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
      Subunit structure: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits.
      Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Molecular WeightAurora C MW = 36kDa, INCENP MW = 38kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250µg size as catalog # 14-911M - Call for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      14-911 04053252290879