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07-164-AF647 Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate

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07-164-AF647
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue Number07-164-AF647
      DescriptionAnti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Histone H2A.X
      • Histone H2A.X
      • H2a/x
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis AlexaFluor®647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.
      Key Applications
      • Immunocytochemistry
      Application NotesSee data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).
      EpitopePhosphorylated Ser139
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes Histone H2A.X phosphorylated at Serine 139.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Vertebrates based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFX
      • H2AX
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~15 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunocytochemistry Analysis:

      A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      07-164-AF647 04053252983672

      Documentation

      Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor® 647 Conjugate Analysenzertifikate

      TitelChargennummer
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate 3033499
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate 2846384
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - Q2443132 Q2443132
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3185032 3185032
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3508342 3508342
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3819095 3819095
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 3944550 3944550
      Anti-phospho H2A.X (Ser139), Alexa Fluor® 647 Conjugate - 4081596 4081596

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies