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MABE56 Anti-pan Ago Antibody, clone 2A8

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MABE56
100 µg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE56
      DescriptionAnti-pan Ago Antibody, clone 2A8
      Alternate Names
      • eukaryotic translation initiation factor 2C, 2
      • protein argonaute-2
      • argonaute 2
      • Protein slicer
      • argonaute2
      • PAZ Piwi domain protein
      • Eukaryotic translation initiation factor 2C
      • Argonaute2
      • Argonaute4
      • Ago4
      • Protein argonaute-4
      Background InformationArgonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-pan Ago Antibody, clone 2A8 is a Mouse Monoclonal Antibody for detection of pan Ago also known as protein argonaute-2 or PAZ Piwi domain protein & has been validated in WB, IP, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
      Biological Information
      ImmunogenHistidine - tagged recombinant protein corresponding to human Ago.
      Clone2A8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • EIF2C2l
      • AGO2
      • Q10
      • PPD
      • eIF-2C 2
      • hAgo2
      • Argonaute1
      • Ago1
      • eIF-2C 1
      • EIF2C1
      • AGO1
      • Argonaute3
      • AGO3
      • EIF2C3

      • EIF2C4
      • : AGO4
      • KIAA1567
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

      CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester.

      ENZYME REGULATION: Inhibited by EDTA.

      SUBUNIT STRUCTURE: Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, EIF2C2/AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with EIF2C1/AGO1. Also interacts with DDB1, DDX6, DDX20, DDX47, DHX9, DHX36, EIF6, FXR1, GEMIN4, ILF3, MOV10, PRMT5, P4HA1, P4HB, SART3, TNRC6A, TNRC6B and UPF1. Interacts with the P-body components DCP1A and XRN1.

      SUBCELLULAR LOCATION: Cytoplasm › P-body. Nucleus. Note: Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

      DOMAIN: The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).

      PTM: Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.

      SEQUENCE SIMILARITIES: Belongs to the argonaute family. Ago subfamily.

      Contains 1 PAZ domain.

      Contains 1 Piwi domain.

      BIOPHYSIOCHEMICAL PROPERTIES: Kinetic parameters:

      KM=1.1 nM for a synthetic 21-nucleotide single-stranded RNA Ref.7 Ref.18

      SEQUENCE CAUTION: The sequence AAH07633.1 differs from that shown. Reason: Erroneous initiation.

      The sequence AAL76093.1 differs from that shown. Reason: cDNA contains a duplication of an internal sequence at the 5' end.

      The sequence BC125214 differs from that shown. Reason: Frameshift at position 450.
      Molecular Weight ~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
      Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Ago on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE56 04053252642548

      Documentation

      Anti-pan Ago Antibody, clone 2A8 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-pan Ago Antibody, clone 2A8 Analysenzertifikate

      TitelChargennummer
      Anti-CK1, clone 3C10F7, Monoclonal Antibody VP1611016
      Anti-pan Ago, clone 2A8 - 2146028 2146028
      Anti-pan Ago, clone 2A8 - 2324663 2324663
      Anti-pan Ago, clone 2A8 - 2370853 2370853
      Anti-pan Ago, clone 2A8 - 2461592 2461592
      Anti-pan Ago, clone 2A8 - 1953035 1953035
      Anti-pan Ago, clone 2A8 - 1975950 1975950
      Anti-pan Ago, clone 2A8 - 1999462 1999462
      Anti-pan Ago, clone 2A8 - 2005945 2005945
      Anti-pan Ago, clone 2A8 - 2026422 2026422

      Literatur

      ÜbersichtAnwendungPub Med ID
      Identification of RISC-associated adenoviral microRNAs, a subset of their direct targets, and global changes in the targetome upon lytic adenovirus 5 infection.
      Bellutti, F; Kauer, M; Kneidinger, D; Lion, T; Klein, R
      Journal of virology  89  1608-27  2015

      Abstract anzeigen
      25410853 25410853
      Characterization of the mammalian miRNA turnover landscape.
      Guo, Y; Liu, J; Elfenbein, SJ; Ma, Y; Zhong, M; Qiu, C; Ding, Y; Lu, J
      Nucleic acids research  43  2326-41  2015

      Abstract anzeigen
      25653157 25653157
      Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis.
      Moore, MJ; Zhang, C; Gantman, EC; Mele, A; Darnell, JC; Darnell, RB
      Nature protocols  9  263-93  2014

      Abstract anzeigen
      24407355 24407355
      Small RNA expression from the human macrosatellite DXZ4.
      Pohlers, M; Calabrese, JM; Magnuson, T
      G3 (Bethesda, Md.)  4  1981-9  2014

      Abstract anzeigen
      25147189 25147189
      miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.
      Zhang, Y; Yang, P; Sun, T; Li, D; Xu, X; Rui, Y; Li, C; Chong, M; Ibrahim, T; Mercatali, L; Amadori, D; Lu, X; Xie, D; Li, QJ; Wang, XF
      Nature cell biology  15  284-94  2013

      Abstract anzeigen
      23396050 23396050
      Efficient and specific gene knockdown by small interfering RNAs produced in bacteria.
      Huang, L; Jin, J; Deighan, P; Kiner, E; McReynolds, L; Lieberman, J
      Nature biotechnology  31  350-6  2013

      Abstract anzeigen
      23475073 23475073
      tRNA-derived microRNA modulates proliferation and the DNA damage response and is down-regulated in B cell lymphoma.
      Maute, RL; Schneider, C; Sumazin, P; Holmes, A; Califano, A; Basso, K; Dalla-Favera, R
      Proceedings of the National Academy of Sciences of the United States of America  110  1404-9  2013

      Abstract anzeigen
      23297232 23297232
      EBV and human microRNAs co-target oncogenic and apoptotic viral and human genes during latency.
      Riley, KJ; Rabinowitz, GS; Yario, TA; Luna, JM; Darnell, RB; Steitz, JA
      The EMBO journal  31  2207-21  2011

      Abstract anzeigen
      22473208 22473208
      Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.
      Saikia, M; Krokowski, D; Guan, BJ; Ivanov, P; Parisien, M; Hu, GF; Anderson, P; Pan, T; Hatzoglou, M
      The Journal of biological chemistry  287  42708-25  2011

      Abstract anzeigen
      Immunoprecipitation23086926 23086926

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies