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ABE213 Anti-monomethyl-Histone H3 (Lys79) Antibody

Use Anti-monomethyl-Histone H3 (Lys79) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, PIA, IP to detect monomethyl-Histone H3 (Lys79) also known as H3K79me1, Histone H3 (mono methyl K79).

Anti-monomethyl-Histone H3 (Lys79) Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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ABE213
200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, DB, PIA, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE213
      DescriptionAnti-monomethyl-Histone H3 (Lys79) Antibody
      Alternate Names
      • Histone H3.1t
      • H3/g
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-monomethyl-Histone H3 (Lys79) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, PIA, IP to detect monomethyl-Histone H3 (Lys79) also known as H3K79me1, Histone H3 (mono methyl K79).
      Key Applications
      • Western Blotting
      • Dot Blot
      • Peptide Inhibition Assay
      • Immunoprecipitation
      Application NotesDot Blot Analysis: A representative lot detected monomethyl-Histone H3 in Dot Blot.

      Peptide Inhibition Analysis: A representative lot blocked monomethyl-Histone H3 in HeLa acid extract.

      Immunoprecipitation Analysis: A representative lot detected monomethyl-Histone H3 in HeLa acid extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 monmethylated at Lys79.
      EpitopeMethylated at Lys79.
      HostRabbit
      SpecificityThis antibody recognizes Histone H3 methylated at Lys79. Using peptide dot blot analysis slight cross-reactivity with dimethyl-Histone H3 (Lys79) and unmodified Histone H3 (Lys79) may be observed. Using peptide inhibition analysis assay no cross reactivity to these peptides is observed.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with multiple vertebrate species due to sequence conservation.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      Modifications
      • Methylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus. Chromosome.

      TISSUE SPECIFICITY: Expressed in testicular cells.
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: A 1:500 dilution of this antibody detected monomethyl-Histone H3 in HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE213 04053252427176