Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-asymmetrically methylated TLS/FUS, clone 2B12, Cat. No. MABE1908, is a mouse monoclonal antibody that detects asymmetrically methylated TLS and is tested for use in and Dot Blot, Immunoprecipitation, and Western Blotting.
More>>Anti-asymmetrically methylated TLS/FUS, clone 2B12, Cat. No. MABE1908, is a mouse monoclonal antibody that detects asymmetrically methylated TLS and is tested for use in and Dot Blot, Immunoprecipitation, and Western Blotting. Less<<
RNA-binding protein FUS (UniProt: P35637; also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Arginine methylation, carried out by protein arginine methyltransferases (PRMT), can be monomethylated or demethylated. Dimethylation can occur both in symmetric (me2s) and asymmetric (me2a) manner. Asymmetric demethylation is catalyzed by the type I class of PRMTs (PRMT1, 3, 4, 6 and 8), and symmetric dimethylarginine (sDMA) is catalyzed by the type II class (PRMT5 and 7). Fused in sarcoma/ translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. It may also play a role in maintenance of genomic integrity. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. FUS is reported to be involved in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Clone 2B12 selectively detects the asymmetrically dimethylated TLS. (Ref.: Fujimoto, K., and Kurokawa, R. (2014). Cell Biosci. 4; 77; Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-asymmetrically methylated TLS/FUS, clone 2B12, Cat. No. MABE1908, is a mouse monoclonal antibody that detects asymmetrically methylated TLS and is tested for use in and Dot Blot, Immunoprecipitation, and Western Blotting.
Key Applications
Dot Blot
Immunoprecipitation
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected asymmetrically methylated TLS/FUS in Western Blotting applications (Cui, W., et. al. (2018). J Biol Chem. 293(28):10937-10948; Fujimoto, K., et. al. (2014). Cell Biosci. 4:77).
Dot Blot Analysis: A representative lot detected asymmetrically methylated TLS/FUS in Dot Blot applications (Fujimoto, K., et. al. (2014). Cell Biosci. 4:77).
Immunoprecipitation Analysis: A representative lot detected asymmetrically methylated TLS/FUS in Immunoprecipitation applications (Fujimoto, K., et. al. (2014). Cell Biosci. 4:77; Cui, W., et. al. (2018). J Biol Chem. 293(28):10937-10948).
Dot Blot Analysis: A 1:100 dilution from a representative lot detected asymmetrically methylated TLS/FUS in TLS peptides that were either asymmetrically modified or symmetrically modified arginines (Courtesy of Riki Kurokawa, Ph.D., Saitama Medical University, Hidaka, Saitama Japan)
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Synthetic peptide corresponding to CGGRme2aGRme2aGGSG (R216/R218me2a).
Epitope
N-terminal half, asymmetric dimethylarginine
Clone
2B12
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 2B12 is a mouse monoclonal antibody that specifically detects asymmetrically methylated TLS/FUS.
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Hamster and Mouse based on 100% sequence homology.
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Arginine methylation of translocated in liposarcoma (TLS) inhibits its binding to long noncoding RNA, abrogating TLS-mediated repression of CBP/p300 activity. Cui W, Yoneda R, Ueda N, Kurokawa R. J Biol Chem
293(28)
10937-10948
2018
Translocated in liposarcoma (TLS) is an RNA-binding protein and a transcription-regulatory sensor of DNA damage. TLS binds promoter-associated noncoding RNA (pncRNA) and inhibits histone acetyltransferase (HAT) activity of CREB-binding protein (CBP)/E1A-binding protein P300 (p300) on the cyclin D1 (CCND1) gene. Although post-translational modifications of TLS, such as arginine methylation, are known to regulate TLS's nucleocytoplasmic shuttling and assembly in stress granules, its interactions with RNAs remain poorly characterized. Herein, using various biochemical assays, we confirmed the earlier observations that TLS is methylated by protein arginine methyltransferase 1 (PRMT1) in vitro The arginine methylation of TLS disrupted binding to pncRNA and also prevented binding of TLS to and inhibition of CBP/p300. This result indicated that arginine methylation of TLS abrogates both binding to pncRNA and TLS-mediated inhibition of CBP/p300 HAT activities. We also report that an arginine residue within the Arg-Gly-Gly domain of TLS, Arg-476, serves as the major determinant for binding to pncRNA. Either methylation or mutation of Arg-476 of TLS significantly decreased pncRNA binding and thereby prevented a pncRNA-induced allosteric alteration in TLS that is required for its interaction with CBP/p300. Moreover, unlike WT TLS, an R476A TLS mutant did not inhibit CCND1 promoter activity in luciferase reporter assays. Taken together, we propose the hypothesis that arginine methylation of TLS regulates both TLS-nucleic acid and TLS-protein interactions and thereby participates in transcriptional regulation.
Development of a mouse monoclonal antibody for the detection of asymmetric dimethylarginine of Translocated in LipoSarcoma/FUsed in Sarcoma and its application in analyzing methylated TLS. Fujimoto K, Kurokawa R. Cell Biosci
4
77
2014
RNA-binding protein Translocated in LipoSarcoma/FUsed Sarcoma (TLS/FUS) is one of causative genes for familial amyotrophic lateral sclerosis (ALS). We previously identified that TLS was associated with protein arginine methyltransferase 1 (PRMT1), and four arginine residues within TLS (R216, R218, R242 and R394) were consistently dimethylated. Protein arginine methylation is involved in various cellular events such as signal transduction, transcriptional regulation and protein-protein interactions.
