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This rat monoclonal Anti-alpha-Tubulin Antibody, clone YOL1/34, Cat. No. CBL270-I is validated for use in Affinity Binding Assay, Electron Microscopy, ELISA, Immunocytochemistry, and Western Blotting for the detection of alpha-tubulin.
More>>This rat monoclonal Anti-alpha-Tubulin Antibody, clone YOL1/34, Cat. No. CBL270-I is validated for use in Affinity Binding Assay, Electron Microscopy, ELISA, Immunocytochemistry, and Western Blotting for the detection of alpha-tubulin. Less<<
Anti-alpha-Tubulin Antibody, clone YOL1/34: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Tubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast. Microtubules are dynamic protein filaments involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Microtubules are assembled from α- and β-tubulin subunits, both of which are subjected to various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation. These PTMs can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins. Because the C-terminus tyrosine/phenylalanine is preceded by glutamate (E) residues, the detyrosinated/dephenylalaninated α-tubulin is also referred to as Glu-tubulin. While tyrosine can be reattached to the C-terminus of the Glu-tubulin by tubulin-tyrosine ligase in mammals, phenylalanine is not added posttranslationally to the Glu-tubulin in yeast despite the presence of the yeast YBR094w gene that exhibits significant homology to tubulin-tyrosine ligase in other organisms
References
Product Information
Format
Purified
Presentation
Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This rat monoclonal Anti-alpha-Tubulin Antibody, clone YOL1/34, Cat. No. CBL270-I is validated for use in Affinity Binding Assay, Electron Microscopy, ELISA, Immunocytochemistry, and Western Blotting for the detection of alpha-tubulin.
Key Applications
Affinity Binding Assay
Electron Microscopy
ELISA
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected alpha-tubulin in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL from a representative lot detected alpha-tubulin in 10 µg of NIH/3T3 mouse fibroblast and PC12 rat pheochromocytoma cell lysates.
Affinity Binding Assay: Pig brain α-tubulin, α-tubulin/β-tubulin heterodimer, as well as α-tubulin proteolytic fragments a.a. 414-425 and a.a. 403-422, but not β-tubulin or actin, effectively competed against immobilized α-tubulin for YOL 1/34 binding (Breitling, F., and Little, M. (1986). J. Biol. Chem. 189(2):367-370).
Electron Microscopy Analysis: A representative lot immunostained microtubules of crude yeast nuclear envelope preparation (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).
ELISA Analysis: A representative lot detected pig brain α-tubulin by direct (non-sandwich) ELISA (Breitling, F., and Little, M. (1986). J. Biol. Chem. 189(2):367-370).
Immunocytochemistry Analysis: A representative lot immunostained fixed NIH/3T3 cells as well as yeast spheroplasts and nuclei preparations (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).
Immunocytochemistry Analysis: A representative lot immunostained microtubules of fixed Dictyostelium (amoeba) as well as isolated and stabilized amoeba cytoskeleton (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).
Western Blotting Analysis: A representative lot detected α-tubulin in spermatozoa sample from from fertile and infertile men (Breitling, F., et al. (1991). Gene. 104(2):147-153).
Biological Information
Immunogen
Yeast tubulin (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).
Clone
YOL1/34
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
Clone YOL1/34 reacts with alpha-tubulin, but not beta-tubulin, by targeting a C-terminal epitope highly conserved among alpha-tubulin subtypes. Epitope is not present in alpha-tubulin 4B (TUBA4B; UniProt Q9H853).
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Porcine
Rat
Yeast
Species Reactivity Note
Human, Mouse, Porcine, Rat, Amoeba, Yeast. Exprected to react with a broad range of species based on 100% sequence homology.
~50 kDa observed. 50.14/46.30 kDa (human alpha-1A isoform 1/2; TUBA1A; TUBA3), 50.15/37.22 kDa (human alpha-1B isoform 1/2; TUBA1B), 44.96 kDa (human alpha-2; TUBA2; TUBA3C; TUBA3D), 49.92/48.33 kDa (human alpha-4A isoform 1/2; TUBA4A; TUBA1), 50.14 kDa (mouse & rat alpha-1A; Tuba1a; Tuba1), 50.15 kDa (mouse & rat alpha-1B; Tuba1b; Tuba2), 49.96 kDa (mouse & rat alpha-3; Tuba3; Tuba3a; Tuba3b), 49.92 kDa (mouse & rat alpha-4A; Tuba4a; Tuba4), 49.80 kDa (yeast) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected alpha-tubulin in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
