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Anti-alpha TLR10, clone 5C2C5, Cat. No. MABF2094, is a mouse monoclonal antibody that detects Toll-like receptor 10 and has been tested for use in Flow Cytometry and Inhibition/Functional Analysis.
More>>Anti-alpha TLR10, clone 5C2C5, Cat. No. MABF2094, is a mouse monoclonal antibody that detects Toll-like receptor 10 and has been tested for use in Flow Cytometry and Inhibition/Functional Analysis. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2094-100UG
Description
Anti-alpha TLR10 Antibody, clone 5C2C5
Alternate Names
CD290
Background Information
Toll-like receptor 10 (UniProt: Q9BXR5; also known as CD290) is encoded by the TLR10 (also known as UNQ315/PRO358) gene (Gene ID: 81793) in human. Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. The extracellular N-terminal region of TLRs recognizes specific pathogen components. At least 10 different members of TLR family have been identified in humans that detect different pathogen associated molecular patterns (PAMPs), including lipopolysaccharides, flagellin, bacterial CpG DNA, and viral RNA and DNA. Recognition of PAMPs by TLRs is considered as a key process for the induction of an inflammatory response. TLR10 is a single-pass type I membrane protein that is highly expressed in spleen, lymph node, thymus, and tonsil. Higher expressions have also been reported in promyelocytic HL-60 and in B-cell lines. It acts via MYD88 and TRAF6, leading to cytokine secretion and the inflammatory response. In TLR10-expressing cells, IkappaB alpha is shown to be consistently inhibited suggesting that TLR10 suppresses NF-kB signaling upstream of IkappaB alpha degradation. TLR10 is synthesized with a signal peptide (aa 1-19) that is cleaved off in the mature form, which contains an extracellular domain (aa 20-576), a transmembrane domain (aa 577-597), and a cytoplasmic domain (aa 598-811). Clone 5C2C5 is shown to suppress LPS-induced IL-6 production in isolated monocytes. (Ref: Hess, NJ., et al. (2017). J. Leukoc. Biol. 101(5); 1245-1252; Jiang, S., et al. (2016). J. Immunol. 196(9): 3834-3841).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
Application
Anti-alpha TLR10, clone 5C2C5, Cat. No. MABF2094, is a mouse monoclonal antibody that detects Toll-like receptor 10 and has been tested for use in Flow Cytometry and Inhibition/Functional Analysis.
Key Applications
Flow Cytometry
Inhibits Activity/Function
Application Notes
Agonist Analysis: A representative lot activated TLR10 receptor when added 30 minutes prior to stiimulation of monocytes. (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252).
Flow Cytometry Analysis: A representative lot detected alpha TLR10 in Flow Cytometry applications (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252; Hess, N.J., et. al. (2017). J Immunol. 198(2):699-707).
Inhibition/Function Analysis: A representative lot suppressed LPS-induced IL-6 production in isolated monocytes when added after stimulation. (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252).
Biological Information
Immunogen
Recombinant fragment corresponding to 455 amino acids from the N-terminal, extracellular domain of human Toll-like receptor 10.
Epitope
extracellular domain
Clone
5C2C5
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 5C2C5 is a mouse monoclonal antibody that detects human Toll-like receptor 10.
Evaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: 2 µg/mL of this antibody detected alpha TLR10 in one million human peripheral blood mononuclear cells (PBMC).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Toll-like receptors play a central role in the initiation of adaptive immune responses with several TLR agonists acting as known B cell mitogens. Despite thousands of publications on TLRs, the function of TLR10 remains unknown. We have found that Ab-mediated engagement of TLR10 on primary human B cells suppresses B cell proliferation, cytokine production, and signal transduction. When challenged with either a T independent or T dependent Ag, TLR10 transgenic mice exhibit diminished Ab responses. Adoptive transfer of splenic B cells into B cell-deficient mice revealed that the suppressive effects on Ag-specific humoral immune responses are entirely B cell intrinsic. Our results demonstrate that TLR10 has a functional role within the B cell lineage that is distinct from that of other TLR family members and may provide a potential therapeutic target for diseases characterized by dysregulated B cell activity.
TLR10 suppresses the activation and differentiation of monocytes with effects on DC-mediated adaptive immune responses. Hess, NJ; Felicelli, C; Grage, J; Tapping, RI J Leukoc Biol
101
1245-1252
2016
TLRs are important pattern-recognition receptors involved in the activation of innate immune responses against foreign pathogens. TLR10 is the only TLR family member without a known ligand, signaling pathway, or clear cellular function. Previous work has shown that TLR10 suppresses proinflammatory cytokine production in response to TLR agonists in a mixed human mononuclear cell population. We report that TLR10 is preferentially expressed on monocytes and suppresses proinflammatory cytokine production resulting from either TLR or CD40 stimulation. TLR10 engagement affects both the MAPK and Akt signaling pathways, leading to changes in the transcriptome of isolated human monocytes. Differentiation of monocytes into dendritic cells in the presence of an αTLR10 mAb reduced the expression of maturation markers and the induction of proinflammatory cytokines, again in response to either TLR or CD40 stimulation. Finally, in coculture experiments, TLR10 differentiated dendritic cells exhibited a decreased capacity to activate T cells as measured by IL-2 and IFN-γ production. These data demonstrate that TLR10 is a novel regulator of innate immune responses and of the differentiation of primary human monocytes into effective dendritic cells.
