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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.
More>>Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
The Werner’s syndrome helicase (RecQ-like type 3; RecQ3; Exonuclease WRN) is a ubiquitously expressed nuclear protein belonging to the RecQ family. It functions as a helicase and exonuclease enzyme, and plays a crucial role in the formation of DNA replication centers. This protein is also involved in DNA repair and recombination, and may be involved in transcriptional events mediated by RNA polymerase II. It is regulated by phosphorylation and is targeted by the DNA-dependent protein kinase enzyme. Disrupted expression of the gene encoding the Werner’s syndrome helicase protein often results in the production of truncated Werner’s syndrome helicase proteins. Over 50 gene mutations may contribute to this effect—and lead to the development of Werner syndrome, a condition characterized by accelerated aging and early onset of age-related conditions such as cancer, atherosclerosis, and osteoporosis.
References
Product Information
Format
Purified
Control
HEK293 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human WRN.
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.
FUNCTION: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
COFACTOR: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).
SUBUNIT STRUCTURE: Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
INVOLVEMENT IN DISEASE: Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Werner's syndrome helicase (WRN) in 10 µg of HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
