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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.
More>>Anti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting. Less<<
Tropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, Tpm3) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. Tropomyosins are rod-like dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Tropomyosin-3 is a homodimeric protein in slow-twitch skeletal muscle that can also form heterodimers of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain. It binds to actin filaments in muscle and non-muscle cells and plays crucial role in the calcium dependent regulation of vertebrate striated muscle contraction. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Mutations in Tpm3 gene are associated with diseases of skeletal muscle such as nemaline myopathy, distal arthrogryposis, and the cap disease that is characterized by myopathy and hypotonia that progresses over time. Clone 4D10A5 recognizes the mammalian tropomyosin exon 9a present in muscle and cytoskeletal isoforms produced by the TPM1, TPM2 and TPM3 genes. (Ref.: Schevzov, G., et al. (2011). BioArchitecture, 1 (4); 135-164).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-Tropomyosin 3 alpha chain, clone 4D10A5, Cat. No. MABT1336, is a mouse monoclonal antibody that detects Tropomyosin alpha-3 chain and is tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Tropomyosin 3 alpha chain in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
Western Blotting Analysis: A 1:125-500 dilution from a representative lot detected Tropomyosin 3 alpha chain in Mouse kidney, skeletal muscle, and recombinant 9a samples (Courtesy of Dr. Jeff Hook, Ph.D., School of Medical Sciences, UNSW Sydney Australia).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Diphtheria Toxoid Carrier Protein conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of isoform 2 of rat Tropomyosin alpha-3 chain (Tpm 3).
Epitope
C-terminal
Clone
4D10A5
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 4D10A5 is a mouse monoclonal antibody that detects Tropomyosin alpha-3 (Tpm3) chain of isoform 2. It targets and epitope within 27 amino acids from the C-terminal region.
28.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Tropomyosins are rod-like dimers which form head-to-tail polymers along the length of actin filaments and regulate the access of actin binding proteins to the filaments.1 The diversity of tropomyosin isoforms, over 40 in mammals, and their role in an increasing number of biological processes presents a challenge both to experienced researchers and those new to this field. The increased appreciation that the role of these isoforms expands beyond that of simply stabilizing actin filaments has lead to a surge of reagents and techniques to study their function and mechanisms of action. This report is designed to provide a basic guide to the genes and proteins and the availability of reagents which allow effective study of this family of proteins. We highlight the value of combining multiple techniques to better evaluate the function of different tm isoforms and discuss the limitations of selected reagents. Brief background material is included to demystify some of the unfortunate complexity regarding this multi-gene family of proteins including the unconventional nomenclature of the isoforms and the evolutionary relationships of isoforms between species. Additionally, we present step-by-step detailed experimental protocols used in our laboratory to assist new comers to the field and experts alike.
