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MABC1161 Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29)

This Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29) is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Western Blotting, Affinity Binding Assay for the detection of TSPAN8.

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Research Category: Apoptosis & Cancer Research Sub-Category: Adhesion (CAMs) Gene Symbol: TSPAN8, TM4SF3 UniProt Number: P19075
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MABC1161
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), FC, WB, ABAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1161
      DescriptionAnti-TSPAN8 Antibody, clone CO-029 (1116NS-29)
      Alternate Names
      • Tetraspanin-8
      • Transmembrane 4 superfamily member 3
      • Tspan-8
      • Tumor-associated antigen CO-029
      Background InformationTetraspanin-8 (UniProt P19075; also known as Transmembrane 4 superfamily member 3, Tspan-8, Tumor-associated antigen CO-029) is encoded by the TSPAN8 (also known as TM4SF3) gene (Gene ID 7103) in human. CO-029 is a member of the transmembrane 4 or tetraspanin family of cell-surface 4-transmembrane glycoproteins involved in the regulation of cell activation, growth, and motility. Tetraspanin-8 (CO-029 antigen) was originally characterized as a tumor-associated antigen expressed in gastric, colorectal, and pancreatic cancer cells and exert tumor progression-promoting activity. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tetraspanin-8 and tetraspanin CD151 with integrin α6β4 and subsequent internalization of this integrin-tetraspanin complex, resultinig in decreased cell-matrix adhesion and increased cell migration. Tetraspanin-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. Human tetraspanin-8 is a four-transmembrane (10-33, 58-72, 84-109, 206-230) protein with both its N- and C-terminal ends (a.a. 1-9 and 231-237) exposed at the cytoplasmic side.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29) is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Western Blotting, Affinity Binding Assay for the detection of TSPAN8.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      • Western Blotting
      • Affinity Binding Assay
      Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected TSPAN8 in Caco-2 human epithelial colorectal adenocarcinoma cells.
      Affinity Binding Assay: A representative lot detected surface CO-029 antigen (TSPAN8) expression among a panel of human tumor cell lines by radioimmune assay (RIA) and mixed-hemadsorption assay (MHA) (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
      Flow Cytometry Analysis: A representative lot detected surface CO-029 antigen (TSPAN8) immunoreactivity predominantly among tumor cells in pancreatic adenocarcinoma tissues-derived cell cultures (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
      Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with those of integrins alpha6 and beta4 by dual fluorescent immunocytochemistry staining of 8.18 human pancreatic cancer cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
      Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with that of integrin beta4 by dual fluorescent immunocytochemistry staining of human pancreatic cancer Capan1 cells, as well as human colon cancer HT29 and WIDR cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
      Immunohistochemistry Analysis: A representative lot detected an upregulated CO-029 antigen (TSPAN8) immunoreactivity in frozen pancreatic adenocarcinoma tissue sections than normal pancreatic tissue sections (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
      Western Blotting Analysis: A representative lot detected CO-029 antigen (TSPAN8) in integrin alpha3 immunoprecipitates from 8.18 human pancreatic cancer cell lysates (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852)
      Western Blotting Analysis: A representative lot detected the endogenous CO-029 antigen (TSPAN8) in SW948 human colon cancer cell lysate as well as the exogenously expressed CO-029 antigen in transfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
      Biological Information
      ImmunogenSW1116 human colorectal carcinoma cells.
      EpitopeExtracellular domain.
      CloneCO-029
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CO-029 (originally 1116NS-29) detected the glycosylated target bands in CO-029 antigen (TSPAN8) cDNA-transfected, but not untransfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TSPAN8
      • TM4SF3
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight26.04 kDa calculated. ~27-34 kDa reported (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837) due to glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human colon cancer tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TSPAN8 in human colon cancer tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABC1161 04055977353044