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Anti-TFF3, clone TFF-286, Cat. No. MABS2053, is a mouse monoclonal antibody that detects Trefoil factor 3 (TFF3) and has been tested for use in ELISA and Western Blotting.
More>>Anti-TFF3, clone TFF-286, Cat. No. MABS2053, is a mouse monoclonal antibody that detects Trefoil factor 3 (TFF3) and has been tested for use in ELISA and Western Blotting. Less<<
Trefoil factor 3 (UniProt: Q07654; also known as TFF3, Intestinal trefoil factor, hITF, Polypeptide P1.B, hP1.B) is encoded by the TFF3 (also known as ITF, TFI) gene (Gene ID: 7033) in human. TFF3 is a small soluble peptide secreted by mucin-producing intestinal epithelial cells and goblet cells. It is also expressed in the uterus, breast, and in some parts of the hypothalamus and pituitary gland. TFF3 is synthesized with a signal peptide (aa 1-35), which is subsequently cleaved off to produce the mature form. It can exist as a monomer or disulfide-linked homodimer. Upon dimerization, the additional 3(10)-helix involving residues 53-55 occur in the TFF3 homodimer that contributes to its distinct structure. The monomeric form is shown to induce cell migration by activating the MAPK pathway independently of the epidermal growth factor and the homodimeric form is shown to have an anti-apoptotic effect on epithelial cells. TFF3 is reported to promote mucosal wound healing in mice and humans and its elevated expression has been linked with gastric adenocarcinoma progression and biliary diseases. TFF3 expression in the intestine is shown to be regulated by food intake and its overexpression is shown to improve glucose tolerance by blocking the expression of gluconeogenic enzymes. TFF3 treatment in animals is reported to reduce fasting insulin levels. (Ref.: Xue, Y., et al. (2013). PLoS One. 8(9); e75240; Ge, H., et al. (2015). PLoS One. 10(6); e0126924; Khummuang, S., et al. (2017). Biological Procedures Online (2017) 19;14).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-TFF3, clone TFF-286, Cat. No. MABS2053, is a mouse monoclonal antibody that detects Trefoil factor 3 (TFF3) and has been tested for use in ELISA and Western Blotting.
Key Applications
ELISA
Western Blotting
Application Notes
ELISA Analysis: A representative lot detected TFF3 in ELISA applications (Khummuang. S., et. al. (2017). Biol Proced Online. 19:14).
Western Blotting Analysis: A representative lot detected TFF3 in Western Blotting applications (Khummuang. S., et. al. (2017). Biol Proced Online. 19:14).
Biological Information
Immunogen
Recombinant human dimeric TFF3.
Clone
TFF-286
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone TFF-286 is a mouse monoclonal antibody that detects human Trefoil factor 3 (TFF3).
~26 kDa observed for His-and thioredoxin tagged recombinant human TFF3; 10.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in recombinant Human Trefoil Factor-3, expressed in E. coli.
Western Blotting Analysis: 2 µg/mL of this antibody detected His-and Thioredoxin tagged recombinant human TFF3.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Production of Monoclonal Antibodies against Human Trefoil Factor 3 and Development of a Modified-Sandwich ELISA for Detection of Trefoil Factor 3 Homodimer in Saliva. Khummuang, S; Phanphrom, W; Laopajon, W; Kasinrerk, W; Chaiyarit, P; Pata, S Biol Proced Online
19
14
2016
Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malignancy. Determination of TFF3 homodimer expression profiles in saliva may lead to new information about oral biology and diseases. The objective of this study was to generate monoclonal antibodies (mAbs) against TFF3 and apply the produced mAbs for the establishment of ELISA for quantification of dimeric TFF3 in saliva.With our modified hybridoma technique, three hybridoma clones producing anti-TFF3 mAbs having IgG isotype were generated. The mAbs were specific for TFF3 with no cross-reactivity to other TFFs. Using the generated mAbs, a modified-sandwich ELISA with high sensitivity for the quantification of dimeric TFF3 in saliva was developed. Using this ELISA, the amount of dimeric TFF3 in saliva could be measured.A modified-sandwich ELISA for the quantification of TFF3 dimeric form was established. The established ELISA will be a valuable tool for facilitating the investigation of the physiological roles and the diagnostic values of TFF3 in oral diseases. The concept of this modified-sandwich ELISA may be applied for the determination of other homodimeric peptides of interest.
