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Anti-Sulf-2, clone 5D5, Cat. No. MABC1794, is a mouse monoclonal antibody that detects Sulf-2 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
More>>Anti-Sulf-2, clone 5D5, Cat. No. MABC1794, is a mouse monoclonal antibody that detects Sulf-2 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABC1794-100UG
Description
Anti-Sulf-2 Antibody, clone 5D5
Alternate Names
Extracellular sulfatase Sulf-2
hSulf-2
Arylsulfatase
EC:3.1.6.13
N-acetylglucosamine-6-sulfatase
Background Information
Extracellular sulfatase Sulf-2 (UniProt: Q8IWU5; also known as hSulf-2, Arylsulfatase, EC:3.1.6.13, N-acetylglucosamine-6-sulfatase) is encoded by the SULF2 (also known as KIAA1247 and UNQ559/PRO1120) gene (Gene ID: 55959) in human. Sulfatase 1 (Sulf-1) and sulfatase 2 (Sulf-2) are heparan sulfatase editing sulfatases that remove 6-O sulfate moiety within heparan sulfate (HS) chains and alter the affinity of the HS chains for growth factors and growth factor receptors. Sulf-2 is synthesized with a signal peptide (aa 1-24) that is subsequently cleaved off to produce the mature protein. Its N-terminal region contains the catalytic domain (aa 1-415) that is followed by a hydrophilic domain (aa 416-715) and the C-terminal region. The hydrophilic domain is necessary for its endoglucosamine-6-sulfatase activity. Glycosylation at serine 583 is shown to negatively regulate its N-acetylglucosamine-6-sulfatase and arylsulfatase activities. Higher expression of Sulf-2 is reported in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney, and placenta. It is detected in the endoplasmic reticulum, Golgi apparatus, and on the cell surface. A secreted form has also been described that is produced after processing by furin. In a majority of hepatocellular carcinomas (HCC) Sulf-2 enhances tumor growth and progression via activation of the Wnt and TGF-β signaling. It has broad oncogenic effects in HCC, and it increase cell growth by upregulating glypican 3 that promotes release of FGF2 and Wnt form glypican 3 to bind to their cognate receptors and enhance receptor tyrosine kinase activity and Wnt/β-catenin signaling. Knockout of Sulf-2 is reported to suppress HCC tumorigenesis. Two isoforms of Sulf-2 have been described that are produced by alternative splicing. (Ref.: Luo, X., et al. (2021). Hepatology. 74(3); 1411-1428; Singer, MS., et al. (2015). Clin. Chim. Acta. 440; 72-78).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Anti-Sulf-2, clone 5D5, Cat. No. MABC1794, is a mouse monoclonal antibody that detects Sulf-2 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Immunoprecipitation
Inhibits Activity/Function
ELISA
Application Notes
Tested Applications
Inhibition Assay: A representative lot of this antibody inhibited the PDGFRβ-YAP pathway and cholangiocarcinoma (CCA) growth in a murine model. Luo, X., et al. (2021). Hepatology.;74(3):1411-1428.
Western Blotting Analysis: A representative lot detected Sulf-2 in Western Blotting applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).
Immunoprecipitation Analysis: A representative lot detected Sulf-2 in Immunoprecipitation applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).
ELISA: A representative lot detected Sulf-2 in ELISA applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
Full-length recombinant human Sulf-2.
Epitope
Unknown
Clone
5D5
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 5D5 is a mouse monoclonal antibody that detects Sulf-2.
~66 kDa for His/GST-tagged recombinant fragment of human Sulf-2; 100.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting with His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).
Western Blotting Analysis: A 1:250 dilution of this antibody detected His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
