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SC1002 Anti-Sox2 Mouse mAb (245610)

This Anti-Sox2 Mouse mAb (245610) is validated for use in Immunoblotting, Immunocytochemistry, Flow Cytometry for the detection of Sox2.

Anti-Sox2 Mouse mAb (245610): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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SC1002
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Preis & Verfügbarkeit

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SC1002-100UG
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      		 Kst.-Ampulle 100 μg
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      Description
      OverviewRecognizes the ~34 kDa Sox2 protein in NTERA-2 cells.
      Catalogue NumberSC1002
      Brand Family Calbiochem®
      References
      ReferencesAvilion, A.A., et al. 2003.Genes Dev. 17, 126.
      Graham, V., et al. 2003.Neuron 39, 749.
      Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
      Kishi, M., et al. 2000.Development 127, 791.
      Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
      Yuan, H., et al. 1995.Genes Dev. 9, 2635.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
      Positive controlNTERA-2 cells
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1-2 µg/ml)
      Immunocytochemistry (10 µg/ml; see comments)
      Flow Cytometry (see comments)
      Application CommentsFor immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 105 cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
      ImmunogenHuman
      Clone245610
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SC1002-100UG 04055977209341

      Documentation

      Anti-Sox2 Mouse mAb (245610) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Sox2 Mouse mAb (245610) Analysenzertifikate

      TitelChargennummer
      SC1002

      Literatur

      Übersicht
      Avilion, A.A., et al. 2003.Genes Dev. 17, 126.
      Graham, V., et al. 2003.Neuron 39, 749.
      Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
      Kishi, M., et al. 2000.Development 127, 791.
      Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
      Yuan, H., et al. 1995.Genes Dev. 9, 2635.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-January-2008 JSW
      ApplicationImmunoblotting (1-2 µg/ml)
      Immunocytochemistry (10 µg/ml; see comments)
      Flow Cytometry (see comments)
      DescriptionProtein G purified mouse monoclonal antibody derived by immunizing mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~34 kDa Sox2 protein.
      BackgroundSox2 is a member of the Sox (SRY-like HMG box) family of transcription factors, which exhibit diverse roles in development. Sox2 plays a role in specifying the first three lineages that are present at egg implantation. In addition, Sox2 functions in regulating proliferation and differentation in the developing peripheral nervous system. As a result of its role in development, Sox2, together with Oct4 and Nanog, is also essential for the propagation of undifferentiated stem cells.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli
      Clone245610
      IsotypeIgG2a
      Specieshuman, mouse
      Positive controlNTERA-2 cells
      FormLyophilized
      FormulationLyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.
      CommentsFor immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton® X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 105 cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.
      Toxicity Standard Handling
      ReferencesAvilion, A.A., et al. 2003.Genes Dev. 17, 126.
      Graham, V., et al. 2003.Neuron 39, 749.
      Stevanovic, M. 2003.Mol. Biol. Rep. 30, 127.
      Kishi, M., et al. 2000.Development 127, 791.
      Uwanogho, D., et al. 1995.Mech. Dev. 49, 23.
      Yuan, H., et al. 1995.Genes Dev. 9, 2635.