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ABE2613-25UL
Sigma-AldrichAnti-SETDB2
This rabbit polyclonal Anti-SETDB2, Cat. No. ABE2613 is tested for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin) for the detection of Histone-lysine N-methyltransferase SETDB2.
More>>This rabbit polyclonal Anti-SETDB2, Cat. No. ABE2613 is tested for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin) for the detection of Histone-lysine N-methyltransferase SETDB2. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
ABE2613-25UL
Description
Anti-SETDB2
Alternate Names
Histone-lysine N-methyltransferase SETDB2
Chronic lymphocytic leukemia deletion region gene 8 protein
Lysine N-methyltransferase 1F
SET domain bifurcated 2
Background Information
Histone-lysine N-methyltransferase SETDB2 (UniProt: Q96T68; also known as Chronic lymphocytic leukemia deletion region gene 8 protein, Lysine N-methyltransferase 1F, SET domain bifurcated 2) is encoded by the SETDB2 (also known as C13orf4, CLLD8, KMT1F) gene (Gene ID: 83852) in human. SETDB2 is a member of the class V-like SAM-binding methyltransferase superfamily that is involved in the left-right axis specification in early development and mitosis. Specifically, it trimethylates lysine 9 of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones and keeps chromatin in a conformation where the promoter is not accessible for transcription factor binding. SETDB2 is reported to contribute to H3K9me3 in both the interspersed repetitive elements and in centromere-associated repeats and plays a role in chromosome condensation and segregation during mitosis. SETDB2 is shown to be induced by STAT1 in response to type I interferon (IFN /β) signalling to suppress the expression of NF-B target genes to attenuate the inflammatory response. In wound macrophages the expression of SETDB2 is also reported to depend on type I interferon/JAK/STAT1 signaling. SETDB2 has been associated with stimulating cell proliferation, especially in cancer tissue. It is reported to promote hyperproliferation and oncogenesis in acute lymphoblastic leukemia by suppressing CDKN2C activity. (Ref.: Lin, C-H., et al. (2018). Cell Rep. 23(4); 1166-1177; Kimball, AS., et al. (2019). Immunity. 51(2); 258-271; Torrano, J., et al. (2019). Clin. Epigenet. 11; Article 43).
References
Product Information
Format
Serum
Presentation
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
This rabbit polyclonal Anti-SETDB2, Cat. No. ABE2613 is tested for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin) for the detection of Histone-lysine N-methyltransferase SETDB2.
Key Applications
Western Blotting
Chromatin Immunoprecipitation (ChIP)
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Applications: A representative lot detected SETDB2 in Immunohistochemistry applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SETDB2 in mouse testis tissue sections.
Western Blotting Analysis: A representative lot detected SETDB2 in Western Blotting applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected SETDB2 in Chromatin Immunoprecipitation applications (Kimball, A.S., et. al. (2019). Immunity. 51(2):258-271).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal region of human Histone-lysine N-methyltransferase SETDB2.
Epitope
Unknown
Concentration
Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects Histone-lysine N-methyltransferase SETDB2. It targets an epitope within the N-terminal region.
~80 kDa observed; 81.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SETDB2 in human heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
