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MABC1592 Anti-SAP-1 Antibody, clone 123

Detect SAP1 using this rat monoclonal Anti-SAP-1 Antibody, clone 123, Cat. No. MABC1592. It has been tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western blotting.

Anti-SAP-1 Antibody, clone 123: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Gene Symbol: Ptprh, Sap-1 UniProt Number: B6ZDS3
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MABC1592
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIHC, WB, IP, IFRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1592
      DescriptionAnti-SAP-1 Antibody, clone 123
      Alternate Names
      • Receptor type protein tyrosine phosphatase sap-1
      • Stomach-cancer–associated protein tyrosine phosphatase 1
      • SAP-1
      Background InformationReceptor type protein tyrosine phosphatase sap-1 (UniProt: B6ZDS3; also known as Stomach-cancer associated protein tyrosine phosphatase 1, SAP-1) is encoded by Ptprh (also known as Sap1) gene (Gene ID: 545902) in murine species. SAP-1 is a receptor-type (R3) protein tyrosine phosphatase that is expressed predominantly in the gastrointestinal tract, most prominently in the microvilli of the brush border in gastrointestinal epithelial cells. SAP-1 has a single catalytic domain in its cytoplasmic region and eight fibronectin type III-like domains in its extracellular region. It is synthesized with a 27 amino acid signal peptide, which is subsequently cleaved. SAP-1 is reported to play a significant role in the regulation of intestinal tumorigenesis and its expression is markedly increased in colon and pancreatic cancers. Ablation of SAP-1 in IL-10-deficient mice display greater severity of colitis in association with up-regulation of mRNAs for various cytokines and chemokines in the colon. The cytoplasmic region of SAP-1 is shown to bind directly to Lck. Over-expression of SAP-1 results in down-regulation of Lck activity that leads to inhibition of T cell receptor-mediated T cell function. (Ref.: Murata, Y et al. (2015). Proc. Natl. Acad. Sci. USA. 112(31):E4264-71).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect SAP1 using this rat monoclonal Anti-SAP-1 Antibody, clone 123, Cat. No. MABC1592. It has been tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected SAP-1 in HEK293A cells transfected with murine SAP-1 versus mock control. (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan).

      Immunofluorescence Analysis: A representative lot detected SAP-1 in Immunofluorescence applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

      Immunofluorescence Analysis: A representative lot detected SAP-1 in ileum from 8-week old WT mouse (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan).

      Immunoprecipitation Analysis: A representative lot detected SAP-1 in Immunoprecipitation applications (Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

      Immunohistochemistry Analysis: A representative lot detected SAP-1 in Immunohistochemistry applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

      Western Blotting Analysis: A representative lot detected SAP-1 in Western Blotting applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).
      Biological Information
      ImmunogenRecombinant fusion protein comprising the extracellular region of mouse SAP-1 (amino acids 1-604) and the Fc region of human IgG.
      Epitopeextracellular domain
      Clone123
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 123 recognizes SAP-1 in HEK293A cells transfected with murine SAP-1. It targets an epitope in the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Not Human.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ptprh
      • Sap-1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~180 kDa observed; 106.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293A cells transfected with murine SAP-1.

      Western Blotting Analysis: 1 µg/mL of this antibody detected SAP-1 in 10 µg of lysate from HEK293A cells transfected with murine SAP-1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABC1592 04054839176463