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MABE286
Sigma-AldrichAnti-Replication Protein A Antibody, clone RPA34-19
Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC.
More>>Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Anti-Replication Protein A Antibody, clone RPA34-19
Alternate Names
Replication protein A 32 kDa subunit
RP-A p32
Replication factor A protein 2
RF-A protein 2
Replication protein A 34 kDa subunit
RP-A p34
Background Information
RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.
References
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-Replication Protein A Antibody, clone RPA34-19 is a Mouse Monoclonal Antibody for detection of Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32 & has been validated in WB, ICC & IHC.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Application Notes
Immunocytochemistry Analysis: A 1:250 dilution from a reprsentative lot detected Replication Protein A in A431 cells.
Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human placental chorionic villi and in human colorectal adenocarcinoma tissue.
FUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3.
SUBCELLULAR LOCATION: Nucleus. Nucleus > PML body. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
POST-TRANSLATIONAL MODIFICATION: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as an hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence.
Molecular Weight
~34 kDa observed. This protein has 3 isoforms: Isoform 1 (~29 kDa), Isoform 2 (~30 kDa), and Isoform 3 (~39 kDa).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
MABE286
04053252689819
Documentation
Anti-Replication Protein A Antibody, clone RPA34-19 SDB
