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ABE440 Anti-RAG-1 Antibody

Detect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP.

Anti-RAG-1 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: RAG1, RNF74 UniProt Number: P15918
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ABE440
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE440
      DescriptionAnti-RAG-1 Antibody
      Alternate Names
      • V(D)J recombination-activating protein 1
      • RAG-1
      • RING finger protein 74
      Background InformationV(D)J recombination-activating protein 1 (RAG-1) is also called RING finger protein 74. RAG-1 is a component of the RAG complex, which mediates the DNA cleavage phase during V(D)J recombination. As part of the RAG complex, RAG1 mediates the DNA-binding and catalyzes the DNA cleavage activities by introducing a double-strand breaks between the recombination signal sequences (RSS) and the adjacent coding segment. RAG-1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3 as well as polyubiquitination of KPNA1. RAG-1 is expressed in maturing lymphoid cells. RAG-1 defects can cause disease states such as: combined cellular and humoral immune defects with granulomas (CHIDG), severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T-B-NK+ SCID), Omenn syndrome (OS), and alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion severe cytomegalovirus infection and autoimmunity (T-CMVA).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected RAG-1 immunopreciptated from 500 µg of HL-60 cell lysate. (Note: It is recommended to immunoprecipitate RAG-1 before performing WB).

      Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected RAG-1 in Jurkat cells.

      DyLight® is a registered trademark of Thermo Fisher Scientific.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human RAG-1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAG1
      • RNF74
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~120 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in HL-60 cell lysate.

      Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RAG-1 in 500 µg of HL-60 cell lysate. Immunopreciptated protein was then probed with 1 µg/mL of the same antibody in WB.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE440 04053252918032