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MABE343-AF488 Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
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MABE343-AF488
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMABE343-AF488
      DescriptionAnti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      This product is a conjugated version of Cat. No. MABE343.
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
      Clone12D10
      Concentration0.5 mg/mL
      HostMouse
      SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightRefer to Cat. No. MABE343 for observed molecular weight information.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Bestellnummer GTIN
      MABE343-AF488 04053252973918