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MABF28 Anti-PAR-3 Antibody, clone 8E8

Anti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA.

Anti-PAR-3 Antibody, clone 8E8: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Infectious Diseases Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: F2RL2, PAR3 UniProt Number: O00254
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MABF28
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HFC, Enzyme AssaysMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF28
      ReplacesMABS174
      DescriptionAnti-PAR-3 Antibody, clone 8E8
      Alternate Names
      • Proteinase-activated receptor 3
      • Coagulation factor II receptor-like 2
      • Thrombin receptor-like 2
      Background InformationPAR-3 (also known as Coagulation factor II receptor-like 2, Thrombin receptor-like 2 or F2RL2) is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR-3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR-3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR-3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR-3 interacts with PRCKI. PAR-3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR-3 interacts with F11R/JAM1. Antibodies against PAR-3 are present in sera from patients with cutaneous T cell lymphomas.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Mouse platelets from washed whole blood (heparinized)
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA.
      Key Applications
      • Flow Cytometry
      • Enzyme Assays
      Application NotesFlow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)

      Activity Assay Analysis (platelet aggregation): A previous lot was used by an independent laboratory in platelet aggregation assay. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human PAR-3.
      Clone8E8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteDemonstrated to react with mouse.
      Predicted to react with human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCoagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3 activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues. [provided by RefSeq].
      Gene Symbol
      • F2RL2
      • PAR3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

      SUBUNIT STRUCTURE: Interacts with INSC/inscuteable and probably GPSM2.

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

      TISSUE SPECIFICTY: Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut. PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

      SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family.

      SEQUENCE CAUTION: The sequence CAD97628.1 differs from that shown. Reason: Frameshift at position 374.
      Molecular Weight40 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse platelets from washed whole blood (heparinized).

      Flow Cytometry Analysis: 2 µg of this antibody detected PAR-3 in mouse platelets from washed whole blood (heparinized).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF28 04053252413568

      Documentation

      Anti-PAR-3 Antibody, clone 8E8 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-PAR-3 Antibody, clone 8E8 Analysenzertifikate

      TitelChargennummer
      Anti-PAR-3, clone 8E8 - 2449224 2449224
      Anti-PAR-3, clone 8E8 - 2051419 2051419
      Anti-PAR-3, clone 8E8 - 2512105 2512105
      Anti-PAR-3, clone 8E8 - 3124158 3124158
      Anti-PAR-3, clone 8E8 - 3230972 3230972
      Anti-PAR-3, clone 8E8 - 3387606 3387606
      Anti-PAR-3, clone 8E8 - 3955344 3955344
      Anti-PAR-3, clone 8E8 - 4036266 4036266
      Anti-PAR-3, clone 8E8 - 4133106 4133106
      Anti-PAR-3, clone 8E8 - NG1895545 NG1895545
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