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AB5995 Anti-Olig3 Antibody

Anti-Olig3 Antibody detects level of Olig3 & has been published & validated for use in WB, IC, IH.

Anti-Olig3 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: OLIG3, BHLHB7, bHLHB7, Oligo3, Bhlhb7 UniProt Number: Q7RTU3
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AB5995
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5995
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Olig3 Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Olig3 Antibody detects level of Olig3 & has been published & validated for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunohistochemistry: 1:200-1:1,000.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 53-66 of Olig3.
      HostRabbit
      SpecificityRecognizes Olig3. The molecular weight of the protein is 27,909 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OLIG3
      • BHLHB7
      • bHLHB7
      • Oligo3
      • Bhlhb7
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q7RTU3 # May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
      SIZE: 272 amino acids; 29358 Da
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      SIMILARITY: SwissProt: Q7RTU3 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB5995 04053252361753