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AB5688 Anti-NeuroD 2 Antibody

Anti-NeuroD 2 Antibody detects level of NeuroD 2 & has been published & validated for use in IC, IH, IH(P) & WB.

Anti-NeuroD 2 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience, Neuronal & Glial Markers Gene Symbol: NEUROD2, NDRF, NeuroD2, MGC26304 UniProt Number: Q15784
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AB5688
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IHC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5688
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NeuroD 2 Antibody
      Alternate Names
      • NDRF
      • NeuroD-related Factor
      • Neurogenic Differentiation Factor 2
      • Ndr2
      • Brain bHLH Protein KW8
      Background InformationNeurogenic differentiation 2, also known as NEUROD2, is a human gene. This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. Diseases associated with NEUROD2 are motor deficits, ataxia, and seizures. NEUROD2 expression is observed mainly in endovascular invasive cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Cerebellum tissue
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NeuroD 2 Antibody detects level of NeuroD 2 & has been published & validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry: 1:200-1:1,000.
      Immunocytochemistry: 1:500 on PC12 cells
      Western blot: 1:200-1:1,000 using ECL.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 23-39 of human NeuroD2.
      HostRabbit
      SpecificityRecognizes NeuroD 2 (NRDL, KW80), a Helix-loop-helix class of transcription factor.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates.
      Gene Symbol
      • NEUROD2
      • NDRF
      • NeuroD2
      • MGC26304
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15784 # Appears to mediate neuronal differentiation.
      SIZE: 382 amino acids; 41361 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein.
      SUBCELLULAR LOCATION: Nucleus (Probable).
      SIMILARITY: SwissProt: Q15784 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight41 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      The antibody must be "rehydrated" prior to use:
      To rehydrate/reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium
      sulfate/PBS solution: 10 mL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 mg of precipitated antibody, the amount of buffer needed would be 50 mL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody can be stored at:
      2-8°C The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°.

      -20°C For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      -70°C For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB5688 04053252581366