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Anti-NMDAR2D, clone 1G9.39A5, Cat. No. MAB5578-I is a mouse monoclonal antibody that detects Glutamate receptor NMDAR2D and is tested for use in Western Blotting.
More>>Anti-NMDAR2D, clone 1G9.39A5, Cat. No. MAB5578-I is a mouse monoclonal antibody that detects Glutamate receptor NMDAR2D and is tested for use in Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MAB5578-I-100UG
Description
Anti-NMDAR2D Antibody, clone 1G9.39A5
Alternate Names
Glutamate receptor ionotropic NMDA 2D
GluN2D
Glutamate [NMDA] receptor subunit epsilon-4
N-methyl D-aspartate receptor subtype 2D
NR2D
Background Information
Glutamate receptor ionotropic, NMDA 2D (UniProt: Q62645; also known as GluN2D, Glutamate [NMDA] receptor subunit epsilon-4, N-methyl D-aspartate receptor subtype 2D, NMDAR2D, NR2D) is encoded by the Grin2d (also known as GluN2D) gene (Gene ID: 24412) in rat. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. The NMDA-receptor-channels display relatively higher Ca2+ permeability than the non-NMDA ionotropic receptors and can be blocked by Mg2+ in a voltage- dependent manner. NMDAR2D is a multi-pass membrane glycoprotein that is a component of the NMDA receptor complex. It functions as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. The heterotetrameric channel is composed of two zeta subunits (GRIN1), and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) in vitro. In vivo, the subunit composition may depend on the expression levels of the different subunits. Channel activation requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. Its expression is prominent during early development and diminishes in the mature nervous system matures. In the adult rat, NMDAR2D is shown to display a highly restricted pattern of expression. It is abundant in midbrain and brainstem structures, whereas in the forebrain it is found in only a small number of neocortical, neostriatal and hippocampal neurons. NMDAR2D is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains two extracellular domains, three cytoplasmic domains, three continuous and one discontinuous transmembrane domains. It glutamate binding sites are localized to amino acids 536-538 and 714-715. (Ref.: Standaert, DG., et al. (1996). Mol. Brain Res. 42(1); 89-102).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-NMDAR2D, clone 1G9.39A5, Cat. No. MAB5578-I is a mouse monoclonal antibody that detects Glutamate receptor NMDAR2D and is tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected NMDAR2D in Western Blotting applications (Shelkar, G.P., et. al. (2019). Sci Rep. 9(1):7572).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Recombinant rat NMDAR2D.
Epitope
C-terminal
Clone
1G9.39A5
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 1G9.39A5 is a mouse monoclonal antibody that detects NMDAR2D.
Isotype
IgG2b
Species Reactivity
Rat
Species Reactivity Note
Rat. Predicted to react with Mouse, Rabbit based on 100% sequence homology.
~170 kDa observed; 143.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Rat brain membrane preparations.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NMDAR2D in Rat brain membrane preparations.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
The GluN2C- and GluN2D-containing NMDA receptors are distinct from GluN2A- and GluN2B-containing receptors in many aspects including lower sensitivity to Mg2+ block and lack of desensitization. Recent studies have highlighted the unique contribution of GluN2C and GluN2D subunits in various aspects of neuronal and circuit function and behavior, however a direct comparison of the effect of ablation of these subunits in mice on pure background strain has not been conducted. Using knockout-first strains for the GRIN2C and GRIN2D produced on pure C57BL/6N strain, we compared the effect of partial or complete ablation of GluN2C and GluN2D subunit on various behaviors relevant to mental disorders. A large number of behaviors described previously in GluN2C and GluN2D knockout mice were reproduced in these mice, however, some specific differences were also observed possibly representing strain effects. We also examined the response to NMDA receptor channel blockers in these mouse strains and surprisingly found that unlike previous reports GluN2D knockout mice were not resistant to phencyclidine-induced hyperlocomotion. Interestingly, the GluN2C knockout mice showed reduced sensitivity to phencyclidine-induced hyperlocomotion. We also found that NMDA receptor channel blocker produced a deficit in prepulse inhibition which was prevented by a GluN2C/2D potentiator in wildtype and GluN2C heterozygous mice but not in GluN2C knockout mice. Together these results demonstrate a unique role of GluN2C subunit in schizophrenia-like behaviors.
