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06-1025
Sigma-AldrichAnti-NET39 Antibody
Anti-NET39 Antibody is a Rabbit Polyclonal Antibody for detection of NET39 has been validated in WB, ICC.
More>>Anti-NET39 Antibody is a Rabbit Polyclonal Antibody for detection of NET39 has been validated in WB, ICC. Less<<
Anti-NET39 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
phosphatidic acid phosphatase type 2 domain containing 3
chromosome 9 open reading frame 67
probable lipid phosphate phosphatase PPAPDC3
Phosphatidic acid phosphatase type 2 domain-containing protein 3
Background Information
NET39 (nuclear envelope transmembrane protein 39) also known as Probable lipid phosphate phosphatase PPAPDC3, has been shown to play a part in regulation of signaling and gene expression. By effecting mTOR signaling, NET39 has a negative regulatory effect on myoblast differentiation. It is predominantly expressed in cardiac and skeletal muscle. During myoblast differentiation, NET39 is highly upregulated. NET39 has been found to be an important part of regulation for myogenesis and might also be involved in muscle homeostasis.
References
Product Information
Format
Affinity Purified
Control
Rat liver tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-NET39 Antibody is a Rabbit Polyclonal Antibody for detection of NET39 has been validated in WB, ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NET39 in A431 and HeLa cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human NET39 at the cytoplasmic domain.
Epitope
Cytoplasmic domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes NET39 at the cytoplasmic domain.
FUNCTION: Plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling. Has no detectable enzymatic activity (By similarity).
SUBUNIT STRUCTURE: Homo and heterooligomer. Interacts with MTOR; controls MTOR-dependent IGF2 expression during myoblast differentiation (By similarity).
SUBCELLULAR LOCATION: Nucleus envelope. Endoplasmic reticulum membrane. Membrane; Multi-pass membrane protein. Note: Both the N- and C-terminal are exposed to the cytoplasm/nucleoplasm (By similarity).
SEQUENCE SIMILARITIES: Belongs to the PA-phosphatase related phosphoesterase family.
SEQUENCE CAUTION: The sequence CAI16274.1 differs from that shown. Reason: Erroneous gene model prediction.
Molecular Weight
~ 29 kDa observed. The band at ~23 kDa is uncharacterized, but may represent a possible isoform of this protein . (Malik, P., et al. (2010).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in rat liver tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NET39 on 10 µg of rat liver tissue lysate. Note: Western blot performed without Tween.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery. Zuleger, N; Boyle, S; Kelly, DA; de las Heras, JI; Lazou, V; Korfali, N; Batrakou, DG; Randles, KN; Morris, GE; Harrison, DJ; Bickmore, WA; Schirmer, EC Genome biology
14
R14
2013
Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells have yet to be widely identified.To search for such proteins, 23 nuclear envelope transmembrane proteins were screened for their ability to promote peripheral localization of human chromosomes in HT1080 fibroblasts. Five of these proteins had strong effects on chromosome 5, but individual proteins affected different subsets of chromosomes. The repositioning effects were reversible and the proteins with effects all exhibited highly tissue-restricted patterns of expression. Depletion of two nuclear envelope transmembrane proteins that were preferentially expressed in liver each reduced the normal peripheral positioning of chromosome 5 in liver cells.The discovery of nuclear envelope transmembrane proteins that can modulate chromosome position and have restricted patterns of expression may enable dissection of the functional relevance of tissue-specific patterns of radial chromosome positioning.
