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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-MerTK, clone Mer590, Cat. No. MABS2246, is a mouse monoclonal antibody that detects Tyrosine-protein kinase Mer (MerTK) and is tested for use in Activity Assay, ELISA, Flow Cytometry, Inhibition, and Western Blotting.
More>>Anti-MerTK, clone Mer590, Cat. No. MABS2246, is a mouse monoclonal antibody that detects Tyrosine-protein kinase Mer (MerTK) and is tested for use in Activity Assay, ELISA, Flow Cytometry, Inhibition, and Western Blotting. Less<<
Tyrosine-protein kinase Mer (UniProt: Q12866; also known as EC:2.7.10.1, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK) is encoded by the MERTK (also known as MER) gene (Gene ID: 10461) in human. MerTK is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-505), a transmembrane domain (aa 506-526), and a cytoplasmic domain (aa 27-999). Its protein kinase domain is localized to amino acids 587-858. MerTK is highly expressed in testis, ovary, prostate, lung, and kidney tissue. Its expression is not observed in normal B- and T-cells but is expressed in several neoplastic B- and T-cell lines. Overexpression of MerTK has also been reported in non-small cell lung cancer (NSCLC). It is involved in the regulation of cell survival, migration, differentiation, and phagocytosis of apoptotic cells. It transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands, including galectin-3, Tubby protein, Tubby-related protein 1, and GAS6, a member of the Axl subfamily of receptor protein-tyrosine kinases. Ligand binding at the cell surface induces autophosphorylation of MerTK on its intracellular domain that provides docking sites for downstream signaling molecules. Its autophosphorylation on tyrosines 749, 753, and 754 in the activation loop imparts full activity. It is also autophosphorylated on tyrosine 872 that leads to recruitment of downstream partners of the signaling cascade such as phospholipase C 2. Clone Mer590 is shown to rapidly reduce total and surface expression of MerTK and prevents its phosphorylation and downstream signaling. It is also shown to enhance carboplatin induced apoptosis in Colo699 cells and diminish glioblastoma cell migration in vitro. (Ref.: Cummings, CT., et al. (2014). Oncotarget, 5(21); 10434-45; Rogers, AEJ., et al. (2012). Oncogene. 31(38): 4171 4181).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Anti-MerTK, clone Mer590, Cat. No. MABS2246, is a mouse monoclonal antibody that detects Tyrosine-protein kinase Mer (MerTK) and is tested for use in Activity Assay, ELISA, Flow Cytometry, Inhibition, and Western Blotting.
Key Applications
Flow Cytometry
Western Blotting
Inhibition
Activity Assay
ELISA
Application Notes
Tested Applications
Western Blotting Analysis: A representative lot detected MerTK in Western Blotting applications (Rogers, A.E.J., et. al. (2012). Oncogene. 31(38):4171-81).
Flow Cytometry Analysis: A representative lot detected MerTK in Flow Cytometry applications. (Cummings, C.T., et. al. (2014). Oncotarget. 5(21):10434-45; Rogers, A.E.J., et. al. (2012). Oncogene. 31(38):4171-81).
Activity Assay: A representative lot of this antibody reduced surface and total MER tyrosine kinase levels in multiple cell lines. (Cummings, C.T., et. al. (2014). Oncotarget. 5(21):10434-45).
ELISA Analysis: A representative lot detected MerTK in ELISA applications (Rogers, A.E.J., et. al. (2012). Oncogene. 31(38):4171-81).
Inhibition Analysis: A representative lot of this antibody inhibited MerTyrosine kinase activity and reduced the migration of Glioblastoma multiforme cells. (Rogers, A.E.J., et. al. (2012). Oncogene. 31(38):4171-81).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Recombinant human MerTK extracellular domain/Fc chimera produced in CHO cells.
Epitope
Extracellular domain
Clone
Mer590
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone Mer590 is a mouse monoclonal antibody that detects human Tyrosine-protein kinase Mer (MerTK). It targets an epitope within the extracellular domain.
Flow Cytometry Analysis: 1 µg of this antibody detected MerTK in one million A549 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
