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MABF935 Anti-MPR300/IGF-2R/CD222 Antibody, clone 2C2

Anti-MPR300/IGF-2R/CD222 Antibody, clone 2C2 is an antibody against MPR300/IGF-2R/CD222 for use in Immunocytochemistry, Immunohistochemistry, Affinity Binding Assay, Western Blotting.

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Research Category: Inflammation & Immunology Research Sub-Category: Vesicular Trafficking Gene Symbol: IGF2R, CIMPR, MPRI UniProt Number: P11717
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MABF935
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, ABA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF935
      DescriptionAnti-MPR300/IGF-2R/CD222 Antibody, clone 2C2
      Alternate Names
      • Cation-independent mannose-6-phosphate receptor
      • 300 kDa mannose 6-phosphate receptor
      • CD222
      • CI Man-6-P receptor
      • CI-MPR
      • IGF-II receptor
      • Insulin-like growth factor 2 receptor
      • Insulin-like growth factor II receptor
      • M6P/IGF2 receptor
      • M6P/IGF2R
      • M6P-R
      • M6PR
      • MPR 300
      Background InformationCation-independent mannose-6-phosphate receptor (UniProt P11717; also known as 300 kDa mannose 6-phosphate receptor, CD222, CI Man-6-P receptor, CI-MPR, IGF-II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, M6P/IGF2R, M6P-R, M6PR, MPR 300) is encoded by the IGF2R (also known as CIMPR, MPRI) gene (Gene ID 3482) in human. Mannose 6-phosphate (M6P) receptors (MPRs) mediate the delivery of newly synthesized, mannose 6-phosphate-bound lysosomal hydrolases from the secretory pathway to the endosomal system. MPRs bind lysosomal hydrolases in the trans-Golgi network (TGN) and unload the enzyme in the endosomal system. There exist a cation-independent CI-MPR (IGF2R) and a smaller CD-MPR that requires divalent cations for lysosomal hydrolases recognition. MPRs also undergo recycling between the endosomal system and the plasma membrane, where CI-MPR mediates the internalization of other ligands, including insulin-like growth factor II (IGF-II). CI-MPR/IGF2R is produced with a signal peptide (a.a. 1-40), the removal of which yields the mature receptor with a large lumenal/extracellular region (a.a. 41-2304), a transmembrane segment (a.a. 2305-2327), and a cytoplasmic domain (a.a. 2328-2491), The extracellular region is composed of 15 P-type carbohydrate recognition domains, also referred to as MRH (mannose 6-phosphate receptor homology) domains. Domains 3 and 9 bind M6P with high affinity and domain 5 binds M6P with weak affinity, while domain 11 binds IGF-II.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MPR300/IGF-2R/CD222 Antibody, clone 2C2 is an antibody against MPR300/IGF-2R/CD222 for use in Immunocytochemistry, Immunohistochemistry, Affinity Binding Assay, Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Affinity Binding Assay
      • Western Blotting
      Application NotesAffinity Binding Assay: Representative lots were labeled with 125I and employed to bind and assess IGF-II receptor (MPR) on the surface of human fibroblasts (van Rahden, V.A., et al. (2012). Hum. Mol. Genet. 21(23):5019-5038; Braulke, T., et al. (1987). J. Cell Biol.;104(6):1735-1742).
      Affinity Binding Assay: A representative lot competed against IGF-II, but not pentamannose 6-phosphate (PMP), for binding immobilized IGF-II receptor (MPR) (Braulke, T., et al. (1988). Biochem. Res. Commun. 150(3):1287-1293).
      Western Blotting Analysis: A representative lot was labeled with 125I and detected the ~300 kDa IGF-II receptor (MPR300) in lysates from HeLa cells and primary human macrophages (Pohl, S., et al. (2010). J. Biol. Chem. 285(31):23936-23944).
      Immunohistochemistry Analysis: A representative lot detected hepatocyte membrane and cytoplasmic M6P/IGF-2R immunoreactivity in normal human liver frozen sections. A reduced immunostaining of hepatocytes restricted to the sinusoidal part of the cell membrane and an increased signals in perisinusoidal cells was seen in cirrhotic livers (Sedlaczek, N., et al. (2003). Br. J. Cancer. 88(5):733-739).
      Immunocytochemistry Analysis: A representative lot immunostained late endosomes in 2% paraformaldehyde-fixed, 0.01% saponin-permeabilized human fibroblasts (Bakker, A.C., et al. (1997). J. Cell Sci. 110 (Pt 18):2227-2238).
      Biological Information
      ImmunogenRecombinant full-length human MPR300/IGF-2R/CD222.
      EpitopeExtracellular (lumenal) domain.
      Clone2C2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2C2 targets the extracellular (lumenal) domain of IGF-II receptor (MPR). Clone 2C2 competed against IGF-II for MPR binding without affecting MPR interaction with pentamannose 6-phosphate (PMP) or beta-hexosaminidase, nor MPR-mediated beta-hexosaminidase endocytosis (Braulke, T., et al. (1988). Biochem. Res. Commun. 150(3):1287-1293; Braulke, T., et al. (1987). J. Cell Biol.;104(6):1735-1742).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IGF2R
      • CIMPR
      • MPRI
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight270.3 calculated. ~300 kDa reported (Pohl, S., et al. (2010). J. Biol. Chem. 285(31):23936-23944).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected MPR300/IGF-2R/CD222 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF935 04055977333329