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Anti-LOXL2, clone GS341, Cat. No. MABE1946, is a mouse monoclonal antibody that detects LOXL2 and is tested for use in ELISA, Electron Microscopy, Inhibition, and Western Blotting.
More>>Anti-LOXL2, clone GS341, Cat. No. MABE1946, is a mouse monoclonal antibody that detects LOXL2 and is tested for use in ELISA, Electron Microscopy, Inhibition, and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABE1946-100UL
Description
Anti-LOXL2 Antibody, clone GS341
Alternate Names
Lysyl oxidase homolog 2
EC: 1.4.3.13
Lysyl oxidase-like protein 2
Lysyl oxidase-related protein 2
Lysyl oxidase-related protein WS9-14
Background Information
Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. The copper-dependent LOX family contain four LOX homologs (LOXL1, LOXL2, LOXL3, and LOXL4). LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 is shown to upregulate in gliomas and drives tumorigenicity by activating autophagy. LOXL2 activity is strongly induced in hypoxia, and it is reported to be a direct transcriptional target of HIF1A. (Ref.: Grossman, M., et al. (2020). Cancer Res; 76(14); 4249-4258; Zhang, Q., et al. (2020). Front. Oncol. 10; 569584; Herranz, N., et al. (2016). FEBS J. 283(23); 4263-4273).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Anti-LOXL2, clone GS341, Cat. No. MABE1946, is a mouse monoclonal antibody that detects LOXL2 and is tested for use in ELISA, Electron Microscopy, Inhibition, and Western Blotting.
Key Applications
Western Blotting
Inhibition
Enzyme Immunoassay (ELISA)
Electron Microscopy
Application Notes
Tested applications
Inhibition: A representative lot of this antibody inhibiter collagen cross-linking in human dermal fibroblasts (HDF).(Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).
Western Blotting Analysis: A representative lot detected LOXL2 in Western Blotting applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).
ELISA Analysis: A representative lot detected LOXL2 in ELISA applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).
Electron Microscopy: A representative lot detected LOXL2 in Electron Microscopy applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
His-tagged recombinant fragment corresponding to 231 amino acids from the C-terminal half of human Lysyl Oxidase homolog 2 (LOXL2).
Epitope
C-terminal half
Clone
GS341
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone GS341 is a mouse monoclonal antibody that detects Lysyl Oxidase homolog 2 (LOXL2). It targets an epitope within the C-terminal half.
80 kDa observed; 86.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting with recombinant Human LOXL2.
Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Human LOXL2.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
