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MABN2619-25UL
Sigma-AldrichAnti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12
Anti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.
More>>Anti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation. Less<<
Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12
Alternate Names
Alpha-2-thiol proteinase inhibitor
Fitzgerald factor
High molecular weight kininogen
HMWK
Williams-Fitzgerald-Flaujeac factor
Background Information
Kininogen-1 (UniProt: P01042; also known as Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor, KNG) is encoded by the KNG1 (also known as BDK, KNG) gene (Gene ID: 3827) in human. Kininogen (KNG) is the precursor protein of the kallikrein-kinin system. KNGs are shown to act as inhibitors of proteases. Kininigen-1 is a secreted protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that can be further cleave into Kininigen-1 heavy chain (HMW; aa 19-380), Kininien-1 light chain (LMW; aa 390-644), T-kinin (aa 376-389), Bradykinin (aa 381-389), lysyl-bradykinin (aa 380-389), and Low molecular weight growth-promoting factor (aa 431-434). Kininogen-1 is reported to exert anti-angiogenic properties and block the proliferation of endothelial cells. It has been identified as a serum biomarker for advanced colorectal adenoma and colorectal cancer. The HMW-kininogen plays a role in blood coagulation by helping to position prekallikrein and factor XI next to factor XII. It is shown to inhibit the thrombin- and plasmin-induced aggregation of thrombocytes. Activation of factor XII triggers HMW-kininogen cleavage by plasma kallikrein, which results in the generation of cleaved high molecular-weight kininogen (HKc) and the release of bradykinin. Higher rate of HMW-kininogen and elevated levels of plasma kallikrein activity has been recorded in the plasma of Alzheimer s disease subjects and changes HMW and cleaved kininogen levels have been significantly correlated with dementia and neuritic plaque scores. Clone 4B12 detects only the cleaved form (LMW) of kininogen (HKc) and not the HMW form (HKi). (Ref.: Yamamoto-Imoto, H., et al (2018). Alzheimer. Dement. (Amst). 10; 480-489; Xu, J., et al. (2018). J. Exp. Clin. Cancer Res. 37; Article 180).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Enzyme Immunoassay (ELISA) Analysis: A representative lot detected Kininogen-1 in ELISA applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Kininogen-1 in Immunoprecipitation applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Kininogen-1.
Clone
4b12
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 4B12 is a mouse monoclonal antibody that detects human Kininogen-1. It targets an epitope within 19 amino acids from the C-terminal region. It recognizes only the cleaved form.
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Kininogen-1 in human kidney tissue sections.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
MABN2619-25UL
04061841440772
Documentation
Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12 SDB
Accumulation of β-amyloid is a pathological hallmark of Alzheimer's disease (AD). β-Amyloid activates the plasma contact system leading to kallikrein-mediated cleavage of intact high-molecular-weight kininogen (HKi) to cleaved high-molecular-weight kininogen (HKc). Increased HKi cleavage is observed in plasma of AD patients and mouse models by Western blot. For potential diagnostic purposes, a more quantitative method that can measure HKc levels in plasma with high sensitivity and specificity is needed.HKi/c, HKi, and HKc monoclonal antibodies were screened from hybridomas using direct ELISA with a fluorescent substrate.We generated monoclonal antibodies recognizing HKi or HKc specifically and developed sandwich ELISAs that can quantitatively detect HKi and HKc levels in human. These new assays show that decreased HKi and increased HKc levels in AD plasma correlate with dementia and neuritic plaque scores.High levels of plasma HKc could be used as an innovative biomarker for AD.
