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MABE1130 Anti-Hsp70/Hsp72 Antibody, clone 2H9

Anti-Hsp70/Hsp72 Antibody, clone 2H9 is an antibody against Hsp70/Hsp72 for use in Western Blotting.

Anti-Hsp70/Hsp72 Antibody, clone 2H9 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chaperones Gene Symbol: HSPA1A, HSP70-1 UniProt Number: Q27975
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MABE1130
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, BWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1130
      DescriptionAnti-Hsp70/Hsp72 Antibody, clone 2H9
      Alternate Names
      • Heat shock 70 kDa protein 1A
      • Heat shock 70 kDa protein 1
      • HSP70.1
      Background InformationHeat shock 70 kDa protein 1A (UniProt Q27975; also known as Heat shock 70 kDa protein 1, HSP70.1) is encoded by the HSPA1A (also known as HSP70-1) gene (Gene ID 281825 & 282254) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and to prevent apoptosis under various stress conditions.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Hsp70/Hsp72 Antibody, clone 2H9 is an antibody against Hsp70/Hsp72 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: Clone 2H9 ascites fluid immunoprecipitated Hsp70/Hsp72 from human U-937 myeloid leukemia cell lysates (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
      Western Blotting Analysis: A representative lot detected Hsp70/Hsp72 expression and siRNA-mediated Hsp70 down-regulation in HeLa cells (Rohde, M., et al. (2005). Genes Dev. 19(5):570-582).
      Western Blotting Analysis: A representative lot detected the heat schock-inducible Hsp70/Hsp72, but not the constitutively expressed HSPA8/Hsc70 (HSC73) in heat shock protein preparations from bovine skeletal muscle and human myocardium tissue (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
      Western Blotting Analysis: A representative lot detected heat shock-induced Hsp70 expression in four human leukemia cell lines (U937, Jurkat, Molt4, HL60), but not in murine myeloma NSO cells (Lasunskaia, E.B., et al. (1997). Apoptosis. 2(2):156-163).
      Biological Information
      ImmunogenBovine skeletal muscle Hsp70/Hsp72.
      Clone2H9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2H9 reacts with the heat schock-inducible HSP72 (HSPA1A), but not the constitutively expressed HSPA8/Hsc70 (HSC73) (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449). Reactivity toward HSPA1B and HSPA2 (UniProt Q27965 and P34933) has not been determined.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPA1A
      • HSP70-1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Hsp70/Hsp72 in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1130 04055977333312