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Anti-GW182, clone 2D6, Cat. No. MABS2147, is a mouse monoclonal antibody that detects Trinucleotide repeat-containing gene 6A protein (GW182) and is tested for use in and Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.
More>>Anti-GW182, clone 2D6, Cat. No. MABS2147, is a mouse monoclonal antibody that detects Trinucleotide repeat-containing gene 6A protein (GW182) and is tested for use in and Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation. Less<<
Trinucleotide repeat-containing gene 6A protein (UniProt: Q8NDV7; also known as CAG repeat protein 26, EMSY interactor protein, GW182 autoantigen, Protein GW1, Glycine-tryptophan protein of 182 kDa, GW182) is encoded by the TNRC6A (also known as CAGH26, KIAA1460, TNRC6) gene (Gene ID: 27327) in human. GW182 is a ubiquitously distributed mRNA binding protein that contains multiple glycine-tryptophan (GW) repeats and an RNA recognition motif near its C-terminus. It is mainly localized in cytoplasmic structures known as GW bodies (GWBs) and plays a role in mRNA processing and in RNA-mediated gene silencing by both miRNA and siRNA. It is required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNA by argonaute proteins. Clone 2D6 is an IgM antibody that is useful in identifying GW182 and GWBs in formalin-fixed and paraffin-embedded tissues after using an antigen retrieval method. It is reported to react with epitopes within amino acids 586-605 and several other non-overlapping epitopes. Mutations in TNRC6A gene are known to cause familial adult myoclonic epilepsy that is characterized by tremulous finger movements and myoclonic jerks of the limbs. (Ref.: Eystathioy, T., et al. (2003). Hybrid Hybridomas 22(2); 79-86; Eystathioy, T., et al. (2002). Mol. Biol. Cell. 13(4); 1338-1351).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-GW182, clone 2D6, Cat. No. MABS2147, is a mouse monoclonal antibody that detects Trinucleotide repeat-containing gene 6A protein (GW182) and is tested for use in and Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.
Key Applications
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated GW182 in Immunoprecipitation applications (Eystathioy, T., et. al. (2003). Hybrid Hybridomics. 22(2):79-86).
Immunocytochemistry Analysis: A 1:20 dilution from a representative lot detected GW182 in HEp-2 cells.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected GW182 in Immunohistochemistry applications (Eystathioy, T., et. al. (2003). Hybrid Hybridomics. 22(2):79-86).
Immunocytochemistry Analysis: A representative lot detected GW182 in Immunocytochemistry applications (Eystathioy, T., et. al. (2003). Hybrid Hybridomics. 22(2):79-86).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
His-tagged recombinant fragment from the internal region of human Glycine-tryptophan protein of 182 kDa (GW182).
Epitope
N-terminal half
Clone
2D6
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 2D6 is a mouse monoclonal antibody that detects Glycine-tryptophan protein of 182 kDa (GW182). It mainly targets an epitope within 20 amino acids from the N-terminal half, but also recognizes several other epitopes.
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgM.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182 Theophany Eystathioy 1 , Edward K L Chan, Michael Mahler, Leeanne M Luft, Mark L Fritzler, Marvin J Fritzler Hybrid Hybridomics
22(2)
79-86
2003
GW182 is a mRNA binding protein characterized by 60 repeats of glycine (G):tryptophan (W) motifs and is localized in cytoplasmic structures referred to as GW bodies (GWBs). Current evidence suggests that this unique protein plays a role in mRNA processing. To enable a more detailed study of GW182 and GWBs in cells and tissues, including their role in mRNA processing, we developed four monoclonal antibodies (MAbs) that bind the human recombinant GW182 protein. These MAbs can be used for Western blot analysis and indirect immunofluorescence (IIF) on cultured cells and tissues. Of special interest, one of the MAbs, 2D6, can be used to identify GW182 and GWBs in formalin-fixed and paraffin-embedded tissues after using an antigen retrieval method (ARM). All the MAbs described in this study immunoprecipitate the GW182 protein. Epitope mapping using overlapping 15-mer peptides representing the full-length GW182 showed that the major antibody-binding domains of these MAbs are distinct. These MAbs are valuable tools for cell biologists and pathologists to study the location and function of the novel GW182 protein in tissue culture cells, as well as cryopreserved or archived tissues.
