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MAB1083 Anti-GFP Antibody, clone 3F8.2

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MAB1083
100 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1083
      DescriptionAnti-GFP Antibody, clone 3F8.2
      Alternate Names
      • Green Fluorescent protein
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      Product Information
      FormatPurified
      Control
      • Western Blot: GFP recombinant protein
      PresentationPurified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).
      Biological Information
      ImmunogenFull length GFP protein.
      EpitopeFull Length
      Clone3F8.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with the 28 kDa GFP protein.
      IsotypeIgG1κ
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteReacts with most common vertebrate proteins containing the GFP tag.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GFP
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

      SUBUNIT: Monomer.

      SPECIFICITY: Photocytes.

      PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

      Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      SIMILARITIES: Belongs to the GFP family.

      Biophysicochemical properties Absorption:

      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

      Abs(max)=395 nm

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      Ontologies
      Molecular Weight28 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on GFP recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      MAB1083 04053252614200