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MABE1047 Anti-Eyes absent homolog 1 Antibody, clone 6A3.1

Anti-Eyes absent homolog 1, clone 6A3.1 Antibody, Cat. No. MABE1047, is a highly specific mouse monoclonal antibody that targets Eyes absent homolog 1 and has been tested foe uae in Immunocytochemistry and Western Blotting.

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Research Category: Epigenetics & Nuclear Function Gene Symbol: EYA1 UniProt Number: Q99502
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MABE1047
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1047
      DescriptionAnti-Eyes absent homolog 1 Antibody, clone 6A3.1
      Alternate Names
      • Eyes absent homolog 1
      • EC:3.1.3.16
      • EC:3.1.3.48
      Background InformationEyes absent homolog 1 (UniProt: Q99502; also known as EC:3.1.3.16; EC:3.1.3.48) is encoded by the EYA1 gene (Gene ID: 2138) in human. Eyes absent homolog 1 belongs to the HAD-like hydrolase superfamily of proteins. It functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Three isoforms of EYA1 protein have been reported that are produced by alternative splicing. It normally localizes at sites of DNA damage at double-strand breaks. In the embryo, it is highly expressed in kidney and at lower levels in brain. In adults, heart and skeletal muscle display higher expression levels and eye and kidney lack any expression. As a tyrosine phosphatase it dephosphorylates Tyr142 of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. Phosphorylation at Try 142 of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. It also displays phosphatase activity with proteins phosphorylated on Serine and Threonine residues. Mutations in EYA1 gene have been linked to branchiootorenal syndrome 1 that is characterized by branchial cleft fistulas or cysts, hearing loss, and renal malformations.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Eyes absent homolog 1, clone 6A3.1 Antibody, Cat. No. MABE1047, is a highly specific mouse monoclonal antibody that targets Eyes absent homolog 1 and has been tested foe uae in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Eyes absent homolog 1 in HeLa, HUVEC, and A431 cells.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 122 amino acids corresponding to internal region of human Eyes absent homolog 1 (EYA1). The immunogen sequence is conserved in isoforms 1 and 2, but not in 3.
      Clone6A3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6A3.1 detects Eyes absent homolog 1 (EYA1) in human. It targets an epitope within 122 amino acids from the internal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EYA1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~65 kDa observed; 64.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse E13-E14 brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Eyes absent homolog 1 in mouse E13-E14 brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1047 04054839412387