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Anti-Exportin-5, clone EX5 4E1, Cat. No. MABE1922, is a rat monoclonal antibody that detects Exportin-5 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
More>>Anti-Exportin-5, clone EX5 4E1, Cat. No. MABE1922, is a rat monoclonal antibody that detects Exportin-5 and is tested for use in ELISA, Immunofluorescence, and Western Blotting. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
MABE1922-25UL
Description
Anti-Exportin-5 Antibody, clone EX5 4E1
Alternate Names
Exp5:Ran-binding protein 21
Background Information
Exportin-5 (UniProt: Q9HAV4; also known as Exp5, Ran-binding protein 21) is encoded by the XPO5 (also known as KIAA1291, RANBP21) gene (Gene ID: 57510) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Exportin-5 mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). Its expression has been reported in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Exportin-5 binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP results in disassembly of the complex and release of the cargo from the export receptor. Exportin-5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. Exportin-5 is shown to be a pre-miRNA-specific export carrier and in this regard, it is reported to contribute to the development and/or progression of malignant melanoma by influencing miRNA transport and maturation. High levels of Exporotin-5 in melanoma cells leads to their enhanced survival, proliferation and metastasis and supports the aggressiveness of melanoma. (Ref.: Ott, CA., et al. (2016). Oncotarget. 7(38); 62292-62304; Bohnsack, MT., et al. (2004). RNA (2004), 10(2); 185-191).
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-Exportin-5, clone EX5 4E1, Cat. No. MABE1922, is a rat monoclonal antibody that detects Exportin-5 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
Key Applications
ELISA
Immunofluorescence
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Exportin-5 in Western Blotting applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).
Immunofluorescence Analysis: A representative lot detected Exportin-5 in Immunofluorescence applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).
ELISA Analysis: A representative lot detected Exportin-5 in ELISA applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Exportin-5.
Epitope
N-terminal half
Clone
EX5 4E1
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rat
Specificity
Clone EX5 4E1 is a rat monoclonal antibody that detects human Exportin-5. It targets an epitope within 15 amino acids from the N-terminal half.
Isotype
IgG1
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Monkey based on 100% sequence homology.
~135 kDa observed; 136.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A375 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Exportin-5 in A375 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells Corinna Anna Ott 1 , Lisa Linck 1 , Elisabeth Kremmer 2 , Gunter Meister 3 , Anja Katrin Bosserhoff Oncotarget
7(38)
62292-62304
2015
Regulation of gene expression via microRNAs is known to promote the development of many types of cancer. In melanoma, miRNAs are globally up-regulated, and alterations of miRNA-processing enzymes have already been identified. However, mis-regulation of miRNA transport has not been analyzed in melanoma yet. We hypothesized that alterations in miRNA transport disrupt miRNA processing. Therefore, we investigated whether the pre-miRNA transporter Exportin-5 (XPO5) was involved in altered miRNA maturation and functional consequences in melanoma. We found that XPO5 is significantly over-expressed in melanoma compared with melanocytes. We showed enhanced XPO5 mRNA stability in melanoma cell lines which likely contributes to up-regulated XPO5 protein expression. In addition, we identified MEK signaling as a regulator of XPO5 expression in melanoma. Knockdown of XPO5 expression in melanoma cells led to decreased mature miRNA levels and drastic functional changes. Our data revealed that aberrant XPO5 expression is important for the maturation of miRNAs and the malignant behavior of melanoma cells. We suggest that the high abundance of XPO5 in melanoma leads to enhanced survival, proliferation and metastasis and thereby supports the aggressiveness of melanoma.
