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Anti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.
More>>Anti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABC1594-25UL
Description
Anti-DR6 (CD358) Antibody, clone 6B6
Alternate Names
Tumor necrosis factor receptor superfamily member 21
Death receptor 6
CD358
Background Information
Tumor necrosis factor receptor superfamily member 21 (UniProt: Q9EPU5; also known as Death receptor 6, DR6, CD358) is encoded by the Tnfrsf21 (also known as Dr6) gene (Gene ID: 94185) in murine species. DR6 is a single-pass type I membrane glycoprotein that is an orphan member of the TNF receptor superfamily. It controls cell death and differentiation in a cell autonomous manner in different cell types. It is ubiquitous expressed and higher expression has been observed in adult spleen, thymus, testis, prostate, ovary, small intestine, colon, brain, lung and kidney tissue. It is also detected in fetal brain, liver and lung. DR6 is expressed at low levels in proliferating progenitors in the spinal cord, but it is highly expressed by differentiating neurons within the spinal cord and adjacent dorsal root ganglia. Expressed by developing neurons as they differentiate and enter a pro-apoptotic state. DR6 promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B and also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. It also participates in neuronal apoptosis in response amyloid peptides derived from amyloid precursor protein. DR6 is reported to be a substrate for ADAM10 and -secretase. DR6 is shown to be involved in peripheral nervous system (PNS) myelination and DR6 knockout mice display precocious myelination in the PNS. DR6 is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 42-349), a transmembrane domain (aa 350-370), and a cytoplasmic domain (aa 371-655). It contains four TNFR-Cys repeats and its death domain is localized to amino acids 415-498. (Ref.: Columbo, A., et al. (2018). EMBO J. 37: e97390).
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected DR6 (CD358) in Western Blotting applications (Colombo, A., et. al (2018. EMBO J. 37(7)).
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Mouse brain tissue lysates from wild type (WT) versus DR6 knock-out (KO) (Courtesy of Dr. Hung-en Hsia, Group of Neuroproteomics (Head: Prof. Dr. Stefan Lichtenthaler), German Center for Neurodegenerative Diseses (DZNE).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Recombinant fragment corresponding to full-length ectodomain region of mouse DR6 with an N‐terminal CD5 signal peptide and a C‐terminal 2XStrepII tag.
Epitope
extracellular domain
Clone
6B6
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rat
Specificity
Clone 6B6 is a rat monoclonal antibody that detects Death Receptor 6. It targets an epitope within the extracellular domain.
Isotype testing: Identity confirmation by isotyping test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
Death receptor 6 (DR6) is an orphan member of the TNF receptor superfamily and controls cell death and differentiation in a cell-autonomous manner in different cell types. Here, we report an additional non-cell-autonomous function for DR6 in the peripheral nervous system (PNS). DR6-knockout (DR6 KO) mice showed precocious myelination in the PNS Using an in vitro myelination assay, we demonstrate that neuronal DR6 acts in trans on Schwann cells (SCs) and reduces SC proliferation and myelination independently of its cytoplasmic death domain. Mechanistically, DR6 was found to be cleaved in neurons by "a disintegrin and metalloprotease 10" (ADAM10), releasing the soluble DR6 ectodomain (sDR6). Notably, in the in vitro myelination assay, sDR6 was sufficient to rescue the DR6 KO phenotype. Thus, in addition to the cell-autonomous receptor function of full-length DR6, the proteolytically released sDR6 can unexpectedly also act as a paracrine signaling factor in the PNS in a non-cell-autonomous manner during SC proliferation and myelination. This new mode of DR6 signaling will be relevant in future attempts to target DR6 in disease settings.
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