Millipore Sigma Vibrant Logo

ABC495 Anti-Caspase 3 Antibody

Anti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.

Anti-Caspase 3 Antibody : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CASP3, CPP32 UniProt Number: P42574
View Products on Sigmaaldrich.com
ABC495
50 μg  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
—
Bitte erfragen
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information

      Sonderaktionen

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC495
      Replaces06-375
      DescriptionAnti-Caspase 3 Antibody
      Alternate Names
      • Caspase-3
      • CASP-3
      • Apopain
      • Cysteine protease CPP32
      • CPP-32
      • Protein Yama
      • SREBP cleavage activity 1
      • SCA-1
      • Caspase-3 subunit p17
      • Caspase-3 subunit p12
      • Caspase 3
      Background InformationCaspase-3 (EC 3.4.22.56; UniProt P42574; also known as Apopain, CASP-3, CPP-32, PARP cleavage protease, SCA-1, SREBP cleavage activity 1, Caspase 3 apoptosis-related cysteine protease, Cysteine protease CPP32, Procaspase3, Protein Yama) is encoded by the CASP3 (also known as CPP32, CPP32B) gene (Gene ID 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is initially produced with a propeptide sequence (a.a. 1-9), the removal of which yields the 268 a.a. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (a.a. 10-175) fragment and the p12 (a.a. 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (a.a. 29-175) subunit. The p17 and p12 dimerizes and forms the active caspase-3 enzyme. Caspase-3 plays a central role in the sequential activation of caspases during the execution phase of cell apoptosis. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Caspase 3 in HeLa, A431, HUVEC, and NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Caspase 3 near the C-terminus.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with pro-caspase 3, the uncleaved/inactive caspase 3 (32 kDa), and the p12 (12 kDa), but not the p17 (17 kDa) subunit of cleaved/active caspase 3.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Non-Human Primate, Canine, Feline, Hamster based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CASP3
      • CPP32
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~32 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.05 µg/mL of this antibody detected Caspase 3 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABC495 04055977294811

      Verwandte Produkte & Anwendungen

      Related Products

      Bestellnummer Beschreibung  
      06-735 Anti-Caspase 3 Antibody Preis & Verfügbarkeit
      AB3623 Anti-Caspase 3 Antibody, active (cleaved) form Preis & Verfügbarkeit
      MAB10753 Anti-Caspase3 (active form) Antibody, clone 3D9.3 Preis & Verfügbarkeit
      MAB4603 Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 Preis & Verfügbarkeit
      MAB4703 Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18 Preis & Verfügbarkeit

      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies