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PC205L
Sigma-AldrichAnti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb
Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
More>>Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections. Less<<
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Description
Overview
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.
Catalogue Number
PC205L
Brand Family
Calbiochem®
Synonyms
Anti-CGRP
Application Data
Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
Free Floating Sections (see application references) Frozen Sections (1:2000-1:4000, ABC detection) Immunofluorescence (1:100-1:200, see application references) Paraffin Sections (see application references; heat pre-treatment required; see comments)
Application Comments
Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
rat α-calcitonin gene related peptide (α-CGRP)
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Mouse
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
PC205L-100UL
04055977209532
Documentation
Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb SDB
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-October-2007 RFH
Synonyms
Anti-CGRP
Application
Free Floating Sections (see application references) Frozen Sections (1:2000-1:4000, ABC detection) Immunofluorescence (1:100-1:200, see application references) Paraffin Sections (see application references; heat pre-treatment required; see comments)
Application Data
Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
Description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Background
Neurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The calcitonin gene peptide superfamily consists of calcitonin, calcitonin gene-related peptide (CGRP), amylin, and adrenomedullin. CGRP is a 37 amino acid neuropeptide that shows widespread distribution and acts as a potent vasodilator. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
Host
Rabbit
Immunogen species
Rat
Immunogen
rat α-calcitonin gene related peptide (α-CGRP)
Isotype
IgG
Species
mouse, rat
Positive control
Rat amygdala or spinal cord
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
≤0.1% sodium azide
Comments
Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
