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AB10547 Anti-Bcl-10 Antibody

Anti-Bcl-10 Antibody detects level of Bcl-10 & has been published & validated for use in WB, IP, IH(P).

Anti-Bcl-10 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Bcl-10, CARMEN, CIPER, CLAP, Cellular-E10, c-E10, cCARMEN, hCLAP, mE10 UniProt Number: O95999
AB10547
200 µg  
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      Übersicht

      Replacement Information
      Replacement Information04-1101 is the recommended replacement for Discontinued catalog number AB10547

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rhesus Macaque, OxWB, IP, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10547
      DescriptionAnti-Bcl-10 Antibody
      Alternate Names
      • B-cell CLL/lymphoma 10
      • CARD containing molecule enhancing NF-kB
      • CARD-containing apoptotic signaling protein
      • CARD-containing molecule enhancing NF-kappa-B
      • CARD-containing proapoptotic protein
      • CARD-like apoptotic protein
      • CED-3/ICH-1 prodomain homologous E10-like regulator
      • Cellular homolog of vCARMEN
      • Mammalian CARD-containing adapter molecule E10
        caspase-recruiting domain-containing protein
      Background InformationB-cell lymphoma/leukemia 10 (Bcl-10) is a protein component of a membrane raft complex located within the cytoplasm. This complex is comprised of several proteins including Bcl-10, IKBKB, DPP4 and CARD11. Bcl-10 contains a single CARD domain that allows it to interact with other CARD-proteins including CARDs 9, 10, 11 and 14. It also forms a strong complex with MALT1. Bcl-10 is a promoter of NF-kappa-b activation through NIK and IKK pathways, pro-caspase-9 activation, and apoptosis. It may also serve a role as an adaptor protein for the upstream complex TNFR1-TRADD-Rip and the downstream complex NIK-IKK-IKAP, as well as being a substrate for MALT1. Expression of Bcl-10 is ubiquitous in tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Raji cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Bcl-10 Antibody detects level of Bcl-10 & has been published & validated for use in WB, IP, IH(P).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a previous lot detected Bcl-10 in human liver cancer tissue.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Bcl-10 from Raji cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Bcl-10 at the CARD domain.
      EpitopeCARD domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Bcl-10 at the CARD domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rhesus Macaque
      • Ox
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
      Predicted to react with rhesus macaque(monkey) and ox based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq].
      Gene Symbol
      • Bcl-10
      • CARMEN
      • CIPER
      • CLAP
      • Cellular-E10
      • c-E10
      • cCARMEN
      • hCLAP
      • mE10
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION:Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.

      SUBUNIT STRUCTURE: Self-associates by CARD-CARD interaction and forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2.

      SUBCELLULAR LOCATION: Cytoplasm › perinuclear region. Note= Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells.

      TISSUE SPECIFICITY:Ubiquitous.

      PTM: Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.

      DISEASE: A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

      Defects in BCL10 are involved in various types of cancer.

      SEQUENCE SIMILARITY: Contains 1 CARD domain.
      Molecular Weight~32 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Raji cell lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Bcl-10 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB10547 04053252642517