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Anti-Actin, clone 5-16 H1C8, Cat. No. MABT1533, is a mouse monoclonal antibody that detects Actin and has been tested for use in ELISA and Western Blotting.
More>>Anti-Actin, clone 5-16 H1C8, Cat. No. MABT1533, is a mouse monoclonal antibody that detects Actin and has been tested for use in ELISA and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABT1533-100UL
Description
Anti-Actin Antibody, clone 5-16 H1C8
Alternate Names
ACT1
Background Information
Actin-1 (UniProt: P0CJ46) is encoded by the ACT1 (also known as AAC1, At2g37620, E13M22.12) gene (Gene ID: 818339) in Arabidopsis thaliana (Mouse-ear cress). Actins are highly conserved globular multi-functional proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. They are essential component of cell cytoskeleton and play an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Actin 1 is considered as one of the reproductive actins. In plants, it is preferentially expressed in mature pollen, pollen tubes, young embryo sac, and organ primordia and little or no reproductive-gene expression is detected in vegetative organs, such as root, stems, leaves, sepals and petals.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgM in PBS without azide.
Applications
Application
Anti-Actin, clone 5-16 H1C8, Cat. No. MABT1533, is a mouse monoclonal antibody that detects Actin and has been tested for use in ELISA and Western Blotting.
Key Applications
ELISA
Western Blotting
Application Notes
ELISA Analysis: A representative lot detected Actin in ELISA applications (Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).
Western Blotting Analysis: A representative lot detected Actin in Western Blotting applications (Kandasamy, M.K., et. al. (2012). Plant Cell. 24(5):2041-57; Kandasamy, M.K., et. al. (1999). Plant J. 18(6):681-91).
Biological Information
Immunogen
Purified actin (ACT1) from Arabidopsis thaliana plant.
Clone
MAb GEa (5-16 H1C8)
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 5-16 H1C8 is a mouse monoclonal antibody that detects Actin in a wide range of species.
Isotype
IgM
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with all eukaryotes based on 100% sequence homology.
~44 kDa observed; 41.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Actin in human skeletal muscle tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The actin gene family of Arabidopsis has eight functional genes that are grouped into two ancient classes, vegetative and reproductive, and into five subclasses based on their phylogeny and mRNA expression patterns. Progress in deciphering the functional significance of this diversity is hindered by the lack of tools that can distinguish the highly conserved subclasses of actin proteins at the biochemical and cellular level. In order to address the functional diversity of actin isovariants, we have used Arabidopsis recombinant actins as immunogens and produced several new anti-actin monoclonal antibodies. One of them, MAb45a, specifically recognizes two closely related reproductive subclasses of actins. On immunoblots, MAb45a reacts strongly with actins expressed in mature pollen but not with actins in other Arabidopsis tissues. Moreover, immunocytochemical studies show that this antibody can distinguish actin filaments in pollen tubes from those in most vegetative tissues. Peptide competition analyses demonstrate that asparagine at position 79 (Asn79) within an otherwise conserved sequence is essential for MAb45a specificity. Actins with the Asn79 epitope are also expressed in the mature pollen from diverse angiosperms and Ephedra but not from lower gymnosperms, suggesting that this epitope arose in an ancestor common to angiosperms and advanced gymnosperms more than 220 million years ago. During late pollen development in angio- sperms there is a switch in expression of actins from vegetative to predominantly reproductive subclasses, perhaps to fulfil the unique functions of pollen in fertilization.
