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ABE1320 Anti-ASXL2

Anti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.

Anti-ASXL2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ASXL2, ASXH2, KIAA1685 UniProt Number: Q76L83
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ABE1320
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1320
      DescriptionAnti-ASXL2
      Alternate Names
      • Putative Polycomb group protein ASXL2
      • Additional sex combs-like protein 2
      Background InformationPutative Polycomb group protein ASXL2 (UniProt: Q76L83; also known as Additional sex combs-like protein 2) is encoded by the ASXL2 (also known as ASXH2, KIAA1685) gene (Gene ID: 55252) in human. ASXL2 is a putative Polycomb group (PcG) protein that acts by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. It is not required to initiate repression, but is essential to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility. ASXL2 is shown to be involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). It also acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. ASXL2 is reported to be essential for haematopoiesis and acts as a haploinsufficient tumor suppressor in leukemia. ASXL2 gene is one of most commonly mutated genes in AML patients bearing the AML1-ETO fusion oncoprotein and loss of ASXL2 is shown to promote AML1-ETO-mediated leukemogenesis. ASXL2 protein contains two LXXLL motifs (aa 303-307 and 887-891), which are required for an association with nuclear receptors. It also contains an atypical PHD-type zinc finger regions (aa 1397-1434). Two isoforms of ASXL2 are reported that are produced by alternative splicing. Mutation in ASXL2 gene have also been linked to the development of Shashi-Pena syndrome that is characterized by delayed psychomotor development, intellectual disability, and macrocephaly, and neonatal feeding difficulties. (Ref.: Micol, JB et al. (2017). Nat. Commun. DOI: 10.1038/ncomms15429).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ASXL2 in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human Additional sex combs-like protein 2 (ASXL2).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Additional sex combs-like protein 2 (ASXL2) in human cells. It targets an epitope within 22 amino acids from the internal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ASXL2
      • ASXH2
      • KIAA1685
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~154 kDa observed; 153.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji nuclear extract.

      Western Blotting Analysis: 2 µg/mL of this antibody detected ASXL2 in 10 µg of Raji nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE1320 04054839412349