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MABE1900-25UL Anti-AKNA Antibody, clone 4F5

Anti-AKNA, clone 4F5, Cat. No. MABE1900, is a rat monoclonal antibody that detects AKNA and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

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MABE1900-25UL
25 µL  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberMABE1900-25UL
      DescriptionAnti-AKNA Antibody, clone 4F5
      Alternate Names
      • Microtubule organization protein AKNA
      • AT-hook-containing transcription factor
      Background InformationMicrotubule organization protein AKNA (UniProt: Q7Z591; also known as AT-hook-containing transcription factor, AKNA) is encoded by the AKNA (also known as KIAA1968) gene (Gene ID: 80709) in human. AKNA is a centrosomal protein that plays a key role in cell delamination by regulating microtubule organization. It is predominantly expressed by lymphoid tissues and its higher expression is observed in the spleen, lymph nodes and peripheral blood leukocytes. Its expression is reported in B- and T-lymphocytes, Natural killer cells and CD1a+CD14- but not CD1a-CD14+ dendritic cells. AKNA localizes to the distal part of the subdistal appendages of the mother centriole in interphase and is also found at the proximal ends of centrioles and along microtubules. It dissociates from centrosomes during M-phase and reassembles at the centrosomes during late telophase and early G1 phase. This dissociation and reassembly are regulated by its phosphorylation. AKNA presence is also reported in subdistal appendages of the mother centriole in specific subtypes of neural stem cells and all basal progenitors and is required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis. Eight different isoforms of AKNA have been described that are produced by alternative splicing. (Ref.: Ortega, GC., et al. (2019) Nature 567(7746); 113-117).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-AKNA, clone 4F5, Cat. No. MABE1900, is a rat monoclonal antibody that detects AKNA and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected AKNA in Immunofluorescence applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

      ELISA Analysis: A representative lot detected AKNA in ELISA applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

      Western Blotting Analysis: A representative lot detected AKNA in Western Blotting applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 11 amino acids from the C-terminal half of human Microtubule organization protein AKNA.
      EpitopeC-terminal half
      Clone4F5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 4F5 is a rat monoclonal antibody that detects Microtubule organization protein AKNA. It targets an epitope within 11 amino acids from the C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AKNA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~155 kDa observed; 155.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected AKNA in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1900-25UL 04065267327808