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176871 Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells

176871
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Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
176871-10UG
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      Kst.-Ampulle 10 μg
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      Description
      OverviewRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
      Catalogue Number176871
      Brand Family Calbiochem®
      SynonymsPeptidyl-Dipetidase A, ACE
      References
      ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
      Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
      Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
      Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
      FormLiquid
      FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥1 unit/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxins: ≤1.0 EU/µg ACE
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Bestellnummer GTIN
      176871-10UG 04055977222982