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UFC910008 Amicon Ultra-15, PLHK Ultracel-PL Membran, 100 kDa

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UFC910008
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      Übersicht

      Replacement Information

      Key Spec Table

      Key ApplicationsNMWLVolumenMin. Konzentratvolumen
      Proteinextraktion, Proteinaufreinigung100 KDa15 mL200 µL
      Beschreibung
      BestellnummerUFC910008
      Markenname
      • Amicon Ultra
      BeschreibungAmicon Ultra-15, PLHK Ultracel-PL Membran, 100 kDa
      HintergrundinformationenAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
      UFC910008 - 8 pack
      UFC910024 - 24 pack
      UFC910096 - 96 pack



      High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
      High retentate recovery of greater than 90%
      Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
      Heat-sealed membrane minimizes downstream extractables
      100% integrity tested for reliable performance
      Convenient sample monitoring with translucent housing and volume gradations
      Direct pipettor sample access eliminates processing step to recover concentrate
      High concentration factors of 80–100X

      Applications:
      Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
      Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
      Concentration of dilute or pre-purified proteins from column eluents
      Desalting, buffer exchange and protein dialysis
      Literatur
      Produktinformationen
      FiltercodePLHK
      FarbcodeTransparent mit rosem Verschluss
      FiltertypUltrafiltration
      NMWL100 KDa
      Quality LevelMQ400
      Anwendungen
      AnwendungUltracel-100 regenerated cellulose membrane, 15 mL sample volume
      Hauptanwendungen
      • Proteinextraktion
      • Proteinaufreinigung
      AnwendungshinweiseSchwingkopfrotor: 4.000 x g; Festwinkelrotor (35°): 5.000 x g
      Biologische Informationen
      SterilitätNicht steril
      Physiochemische Informationen
      ZentrifugenanwendungProtein Concentration and Desalting
      Abmessungen
      Länge12.1 cm (4.807 in.)
      Durchmesser2.97 cm
      Filterfläche7.6 cm²
      Min. Konzentratvolumen200 µL
      Volumen15 mL
      Materialinformationen
      Chemie
      • Regenerated Cellulose (RC)
      Material der Einheit
      • Housing: Styrene/butadiene
      • Röhrchen: Polypropylen
      • Kappe mit Dichtungsmaterial: Polyethylen
      StützmaterialRegenerierte Zellulose
      Angaben zur Toxikologie
      Sicherheitsinformationen gemäß GHS
      Sicherheitshinweise
      Hinweise zum Produktgebrauch
      Lagerungs- und Versandinformationen
      Verpackungsinformationen
      Menge8
      Transportinformationen
      Ergänzende Informationen
      Spezifikationen
      Global Trade Item Number
      Bestellnummer GTIN
      UFC910008 04053252504129

      Documentation

      Broschüre

      Titel
      REFINE PROTEIN PREPARATION. Tools for better protein analysis

      FAQ

      FrageAntwort
      Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
      What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
      How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
      Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
      Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
      Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
      What is the maximum sample volume for Amicon Ultra?
      Amicon Ultra-4Amicon Ultra-15
      Swinging Bucket4ml 15ml
      Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
      3.5ml(23 degree rotor angle)
      What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
      4ml15ml
      Swinging Bucket4000 x g4000 x g
      3500 x g for concentrated/viscous solutions
      Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
      What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
      Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.

      Gebrauchsanleitungen

      Titel
      Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide