Trypsin digested Cytochrom C


Column Chromolith® CapRod® RP-18e 150 x 0.1 mm Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-Chromolith CapRod-Trypsin digested Cytochrom C 2 ?L-480x379-20140214.png
Mobile phase A: 0.1% formic acid in 2% acetonitrile
B: 0.08% formic acid in 80% acetonitrile
Gradient 98% to 60% A in 35 minutes
Flow rate 2 μL/min
Injection volume 0.1 μL
Sample Trypsin digested Cytochrom C (1mg/mL)
Column Chromolith® CapRod® RP-18e HighResolution 150  x 0.1 mm Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-Chromolith CapRod-Trypsin digested Cytochrom C 400 ?L-480x379-20140214.png
Mobile phase A: 0.1% formic acid in 2% acetonitrile
B: 0.08% formic acid in 80% acetonitrile
Gradient 98% to 60% A in 35 minutes
Flow rate 400 nL/min
Injection volume 0.1 μL
Sample Trypsin digested Cytochrom C (1mg/mL)