Separation on Chromolith® CapRod® RP-8 endcapped


Column Chromolith® CapRod® RP-8e 150 x 0.1 mm Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-Chromolith CapRod-Separation on Chromolith CapRod RP-8 endcapped-480x423-20140214.png
Mobile phase A: 100% Water
B: 100% Acetonitrile
Gradient 3 min
1 min
11 min
5 min hold followed with 25 min reequilibration
12% B to 27% B
hold
27% B to 90% B
Flow rate 2 μL/min
Detection Dionex Ultimate 3000 Variable Wavelength Detector; = 240 nm, cell volume 45 nL
Temperature 25°C
Injection volume 0.01 μL of the sample
Pressure 16.6 MPa (2407 psi) 166 bar
Sample 1. Fluoxymesterone
2. Boldenone
3. Methandrostenolone
4. Testosterone
5. Methyltestosterone
6. Boldenone-Acetate
7. Testosterone-Acetate
8. Nandrolone-Propionate
9. Testosterone-Propionate
10. Nandrolone-Phenylpropionate
11. Testosterone-Isocaproate
8.693 min
9.313 min
9.887 min
9.887 min
10.513 min
12.347 min
12.867 min
13.240 min
13.607 min
14.760 min
15.380 min