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420220 iPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem

iPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.

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Sinônimos: N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII

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CAS #Empirical Formula
1226056-71-8C₁₅H₁₃N₅OS

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      Description
      OverviewA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461) and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.
      Catalogue Number420220
      Brand Family Calbiochem®
      SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
      References
      ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
      Lin, T., et al. 2009. Nat. Methods 6, 805.
      Product Information
      CAS number1226056-71-8
      FormTan powder
      Hill FormulaC₁₅H₁₃N₅OS
      Chemical formulaC₁₅H₁₃N₅OS
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationiPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.
      Biological Information
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      420220-10MG 04055977210811

      Documentation

      iPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      iPSC Induction Enhancer, Thiazovivin - CAS 1226056-71-8 - Calbiochem Certificados de análise

      TítuloNúmero do lote
      420220

      Referências

      Visão geral de referência
      Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
      Lin, T., et al. 2009. Nat. Methods 6, 805.
      Ficha de dados

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-April-2012 JSW
      SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
      DescriptionA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461) and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.
      FormTan powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number1226056-71-8
      Chemical formulaC₁₅H₁₃N₅OS
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
      Lin, T., et al. 2009. Nat. Methods 6, 805.