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189730 Streptavidin

Streptavidin: Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.

Sinônimos: Avidin, Streptomyces avidinii

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189730
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189730-1MG
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      Description
      OverviewA high-affinity biotin binding protein.
      Catalogue Number189730
      Brand Family Calbiochem®
      SynonymsAvidin, Streptomyces avidinii
      References
      ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
      Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
      Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
      Roth, J., et al. 1992. Histochemistry 98, 229.
      Sano, T., et al. 1992. Science 258, 120.
      Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
      Srivastava, P., et al. 1992. Cytometry 13, 711.
      Product Information
      CAS number9013-20-1
      Unit of DefinitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
      FormLyophilized solid
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥10 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      189730-1MG 04055977221961

      Documentation

      Streptavidin MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Streptavidin Certificados de análise

      TítuloNúmero do lote
      189730

      Referências

      Visão geral de referência
      Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
      Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
      Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
      Roth, J., et al. 1992. Histochemistry 98, 229.
      Sano, T., et al. 1992. Science 258, 120.
      Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
      Srivastava, P., et al. 1992. Cytometry 13, 711.

      Brochura

      Título
      Bulk Product Guide
      Ficha de dados

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-April-2008 RFH
      SynonymsAvidin, Streptomyces avidinii
      DescriptionA high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
      FormLyophilized solid
      CAS number9013-20-1
      Specific activity≥10 units/mg protein
      Unit definitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
      SolubilityReconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.
      Storage Protect from moisture
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
      Toxicity Standard Handling
      ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
      Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
      Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
      Roth, J., et al. 1992. Histochemistry 98, 229.
      Sano, T., et al. 1992. Science 258, 120.
      Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
      Srivastava, P., et al. 1992. Cytometry 13, 711.